Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 884436..885090 Replicon chromosome
Accession NZ_CP041425
Organism Escherichia coli strain STEC388

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FNE85_RS04340 Protein ID WP_000244777.1
Coordinates 884683..885090 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FNE85_RS04335 Protein ID WP_000354046.1
Coordinates 884436..884702 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE85_RS04310 879601..880343 + 743 Protein_848 SDR family oxidoreductase -
FNE85_RS04315 880405..881838 - 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -
FNE85_RS04320 881883..882194 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FNE85_RS04325 882358..883017 + 660 WP_000250274.1 hemolysin III family protein -
FNE85_RS04330 883213..884193 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
FNE85_RS04335 884436..884702 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FNE85_RS04340 884683..885090 + 408 WP_000244777.1 toxin CptA Toxin
FNE85_RS04345 885130..885651 - 522 WP_032270388.1 flavodoxin FldB -
FNE85_RS04350 885763..886659 + 897 WP_096129014.1 site-specific tyrosine recombinase XerD -
FNE85_RS04355 886684..887394 + 711 WP_161623593.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FNE85_RS04360 887400..889133 + 1734 WP_096129013.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 861521..886659 25138


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T128414 WP_000244777.1 NZ_CP041425:884683-885090 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T128414 NZ_CP041425:884683-885090 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT128414 WP_000354046.1 NZ_CP041425:884436-884702 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT128414 NZ_CP041425:884436-884702 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAAACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References