Detailed information of TA system TA128405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 34006..34565
• Replicon: plasmid pSTEC409_2
• Accession: NZ_CP041424
• Organism: Escherichia coli strain STEC409

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FNE86_RS23550
• Protein ID: WP_032084131.1
• Coordinates: 34290..34565 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FNE86_RS23545
• Protein ID: WP_032084130.1
• Coordinates: 34006..34290 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE86_RS23520	31404..32096	+	693	WP_181573844.1	fimbrial chaperone	-
FNE86_RS23525	32212..32592	+	381	WP_143361631.1	hypothetical protein	-
FNE86_RS23530	32743..33588	+	846	WP_161620589.1	hypothetical protein	-
FNE86_RS23535	33644..33769	-	126	Protein_35	IS256 family transposase	-
FNE86_RS23540	33841..33921	-	81	Protein_36	ATP-binding protein	-
FNE86_RS23545	34006..34290	+	285	WP_032084130.1	ribbon-helix-helix domain-containing protein	Antitoxin
FNE86_RS23550	34290..34565	+	276	WP_032084131.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FNE86_RS23555	34671..34952	+	282	WP_096117502.1	hypothetical protein	-
FNE86_RS23560	35222..35568	+	347	Protein_40	hypothetical protein	-
FNE86_RS23565	35586..36020	-	435	Protein_41	hypothetical protein	-
FNE86_RS23570	36304..37227	-	924	WP_032084040.1	hypothetical protein	-
FNE86_RS23575	38119..38892	-	774	WP_096117503.1	YaiO family outer membrane beta-barrel protein	-
FNE86_RS23580	38894..39475	-	582	WP_096117506.1	acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..81110	81110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10765.45 Da
• Isoelectric Point: 9.9324

>T128405 WP_032084131.1 NZ_CP041424:34290-34565 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFSGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T128405 NZ_CP041424:34290-34565 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTTCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10517.92 Da
• Isoelectric Point: 5.6558

>AT128405 WP_032084130.1 NZ_CP041424:34006-34290 [Escherichia coli]
MQMKNNSAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQTW
ADSLNTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT128405 NZ_CP041424:34006-34290 [Escherichia coli]
ATGCAAATGAAAAACAATAGTGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGATCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGACCTGG
GCAGACAGCCTCAATACTGACAATCCGTTACCGGTGCCTCGCTGA