Detailed information of TA system TA128402

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4135810..4136645
• Replicon: chromosome
• Accession: NZ_CP041422
• Organism: Escherichia coli strain STEC409

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3L5HA82
• Locus tag: FNE86_RS20165
• Protein ID: WP_061090001.1
• Coordinates: 4136268..4136645 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FNE86_RS20160
• Protein ID: WP_032256355.1
• Coordinates: 4135810..4136178 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE86_RS20120	4131087..4131767	+	681	WP_001282912.1	WYL domain-containing protein	-
FNE86_RS20125	4131918..4132595	+	678	WP_161620543.1	hypothetical protein	-
FNE86_RS20130	4132601..4132834	+	234	WP_161620544.1	DUF905 family protein	-
FNE86_RS20135	4132924..4133742	+	819	WP_042970615.1	DUF945 domain-containing protein	-
FNE86_RS20140	4133834..4134319	+	486	WP_000206661.1	antirestriction protein	-
FNE86_RS20145	4134334..4134810	+	477	WP_122997546.1	RadC family protein	-
FNE86_RS20150	4134879..4135100	+	222	WP_000692303.1	DUF987 domain-containing protein	-
FNE86_RS20155	4135119..4135760	+	642	WP_032256353.1	hypothetical protein	-
FNE86_RS20160	4135810..4136178	+	369	WP_032256355.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FNE86_RS20165	4136268..4136645	+	378	WP_061090001.1	TA system toxin CbtA family protein	Toxin
FNE86_RS20170	4136642..4137130	+	489	WP_032256359.1	hypothetical protein	-
FNE86_RS20175	4137148..4137345	+	198	WP_032256361.1	DUF957 domain-containing protein	-
FNE86_RS20180	4137430..4138275	+	846	WP_032256362.1	DUF4942 domain-containing protein	-
FNE86_RS20185	4138346..4139881	+	1536	WP_097680156.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4128758..4150036	21278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14047.00 Da
• Isoelectric Point: 7.8049

>T128402 WP_061090001.1 NZ_CP041422:4136268-4136645 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T128402 NZ_CP041422:4136268-4136645 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCACGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTCTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13715.44 Da
• Isoelectric Point: 5.8808

>AT128402 WP_032256355.1 NZ_CP041422:4135810-4136178 [Escherichia coli]
VSDSLHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128402 NZ_CP041422:4135810-4136178 [Escherichia coli]
GTGTCAGACTCGCTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGCAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A3L5HA82