128401

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 3920029..3921364 Replicon chromosome
Accession NZ_CP041422
Organism Escherichia coli strain STEC409

Toxin (Protein)


Gene name sanaT Uniprot ID F4VMR9
Locus tag FNE86_RS19125 Protein ID WP_000342497.1
Coordinates 3920029..3920976 (-) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID E0J2C6
Locus tag FNE86_RS19130 Protein ID WP_000152741.1
Coordinates 3920969..3921364 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE86_RS19100 3915882..3916598 + 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -
FNE86_RS19105 3916618..3917724 + 1107 WP_001298927.1 N-acetylneuraminate epimerase -
FNE86_RS19110 3917789..3918769 + 981 WP_000991446.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
FNE86_RS19115 3918777..3919427 - 651 WP_001037963.1 HNH endonuclease -
FNE86_RS19120 3919806..3919991 + 186 WP_000066585.1 hypothetical protein -
FNE86_RS19125 3920029..3920976 - 948 WP_000342497.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
FNE86_RS19130 3920969..3921364 - 396 WP_000152741.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
FNE86_RS19135 3921434..3922267 - 834 WP_001192743.1 DUF4942 domain-containing protein -
FNE86_RS19140 3922573..3924129 + 1557 WP_000202821.1 type I restriction-modification system subunit M -
FNE86_RS19145 3924126..3925400 + 1275 WP_001293046.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB 3913824..3925400 11576


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-303)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 35588.79 Da        Isoelectric Point: 4.9737

>T128401 WP_000342497.1 NZ_CP041422:c3920976-3920029 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp

>T128401 NZ_CP041422:c3920976-3920029 [Escherichia coli]
ATGGATGATTTAACCACTCTTTTTTCTGAAGTGGCTGACGCGCTTGGTATTGAGTCTGTCGCAATTGTGGAAAAAGATCA
CTATATCGTTGAGCTTTTGCAATTGCTTAAACCTCTGGTGTTCGATACTCATCAATTGGTGTTTGCCGGAGGAACCGCTC
TGGCAAAATCAGGTGTCGCACTTAATCGCATGTCGGAAGATGTGGATATCAAACTGGTGCCTAAGGATGCATTTCAGGCA
CTTTCCCGCACTCAGCGAAAAAATACCCGTAAAGAAATTGTTCAGGCAGTTTCCAGTGCAATAGCTTCATCGGGTGTGTT
CAATTTTGATGATGATTACCCAAAAGTGACGCGGGATGAATATCGTTATAATGACATACCTGTTCGTTATCCACAGCGAG
TAGTGCAGGCACCTTGTTTGCGTCCTTTTATCCGGCTGGAACTGATGGAAGCGAAGTTACTGGAGCCTTCTGAATTACGG
GATATTCATTCACTGGTAACAGGGATCACGGGAAAAGCGGCTATTGTTTCAGCGTTTCCATGCGCAACTATCATTAGCAC
ACAGGCAGAAAAGCTGGTGGCGATGTTACGTCGTACTGCGGCAGTTGTGCGAGATGTTGAGCGATTTGATGATGCATCGC
TGGTACGTCATATCTACGATACAAACTGTATTGTTGAGACGAGTCATGTTGATATGAGTCTGCTCGTTGATTTTGTTCAA
CAAACTATTGAGCAGGACATTCAGCGTTACGGCAACCAGTACCCGCAGTTTTGCGCGTCTCCAGTTGATGAGCTAAAAAT
GGGACTGGATGAGTTGGCGAACAATCCTCTCTACCAGATGCGCTATCAGCAGTTTGTTTCGCCAATGGTGTATGGAGAGC
GAAAGATCACATGGGATGAGGCTTACTCCAGGTTTAGGAGCTTAGCGCTAGCCATCCTGAATGCTTGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14619.93 Da        Isoelectric Point: 11.6915

>AT128401 WP_000152741.1 NZ_CP041422:c3921364-3920969 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp

>AT128401 NZ_CP041422:c3921364-3920969 [Escherichia coli]
ATGACGATAAAAGCACGCATTCAGTCACGACTGAAACGTTCTAAACGTTACGTATTCACTCGTGACGACTTTAAAGATAT
TGCAGGCTACGATCAGGTTGGTCGGGTATTGCGCGAGCTTGTCAGGGAAGGGCAACTGTTGAAGGTTGGTTATGGCGTGT
ATACCAAAGCACGGCGGAACGGTATTACAGGCAAGGTAATGCCAGCGGCTCCCGGAGGCTCTTCAGCGGTGATTGTCGAA
ACGCTGGAACGCCTGAAAGTACGCTATCGTTTTGCTGGCGCAACGCAGGCATACAATAGCGGTACTTCTACGCAGATCCC
AGCTTCACCCGAGATAAAAACCCCCCGGCGTTTTAAACGCGTATTATCTGTAGGGAACAGCAGATTAAATGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L5BAQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4ZXD4

References