Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 829602..830256 Replicon chromosome
Accession NZ_CP041422
Organism Escherichia coli strain STEC409

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FNE86_RS04050 Protein ID WP_000244777.1
Coordinates 829849..830256 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FNE86_RS04045 Protein ID WP_000354046.1
Coordinates 829602..829868 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE86_RS04020 824771..825514 + 744 WP_033865725.1 SDR family oxidoreductase -
FNE86_RS04025 825571..827004 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
FNE86_RS04030 827049..827360 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
FNE86_RS04035 827524..828183 + 660 WP_000250274.1 hemolysin III family protein -
FNE86_RS04040 828379..829359 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FNE86_RS04045 829602..829868 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FNE86_RS04050 829849..830256 + 408 WP_000244777.1 toxin CptA Toxin
FNE86_RS04055 830296..830817 - 522 WP_001055874.1 flavodoxin FldB -
FNE86_RS04060 830929..831825 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FNE86_RS04065 831850..832560 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FNE86_RS04070 832566..834299 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T128386 WP_000244777.1 NZ_CP041422:829849-830256 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T128386 NZ_CP041422:829849-830256 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT128386 WP_000354046.1 NZ_CP041422:829602-829868 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT128386 NZ_CP041422:829602-829868 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References