Detailed information of TA system TA128382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 20343..20890
• Replicon: plasmid pSTEC711_5
• Accession: NZ_CP041421
• Organism: Escherichia coli strain STEC711

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: FNE87_RS24845
• Protein ID: WP_000969996.1
• Coordinates: 20609..20890 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: FNE87_RS24840
• Protein ID: WP_000079941.1
• Coordinates: 20343..20612 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE87_RS24795	15592..16152	+	561	WP_000139366.1	fertility inhibition protein FinO	-
FNE87_RS24800	16287..16499	+	213	WP_112832261.1	hypothetical protein	-
FNE87_RS24805	16743..17107	+	365	Protein_14	thermonuclease family protein	-
FNE87_RS24810	17107..17300	+	194	Protein_15	DUF2726 domain-containing protein	-
-	17477..17538	-	62	NuclAT_0	-	-
-	17477..17538	-	62	NuclAT_0	-	-
-	17477..17538	-	62	NuclAT_0	-	-
-	17477..17538	-	62	NuclAT_0	-	-
FNE87_RS24815	17525..17731	+	207	WP_178086280.1	type I toxin-antitoxin system Hok family toxin	-
FNE87_RS24820	18014..18262	+	249	WP_000083838.1	replication regulatory protein RepA	-
FNE87_RS24825	18505..18579	+	75	WP_001367777.1	RepA leader peptide Tap	-
FNE87_RS24830	18572..19429	+	858	WP_033562000.1	incFII family plasmid replication initiator RepA	-
FNE87_RS24840	20343..20612	+	270	WP_000079941.1	hypothetical protein	Antitoxin
FNE87_RS24845	20609..20890	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FNE87_RS24850	21154..22326	+	1173	WP_096934598.1	hypothetical protein	-
FNE87_RS24855	22429..23340	+	912	WP_136769172.1	toll/interleukin-1 receptor domain-containing protein	-
FNE87_RS24860	23482..23877	-	396	WP_096934596.1	plasmid partitioning/stability family protein	-
FNE87_RS24865	23877..24427	-	551	Protein_25	plasmid stabilization protein	-
FNE87_RS24870	24421..24642	+	222	WP_077775928.1	hypothetical protein	-
FNE87_RS24875	24664..24999	-	336	WP_161620440.1	colicin transporter	-
FNE87_RS24880	25128..25475	+	348	WP_033812875.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..82344	82344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T128382 WP_000969996.1 NZ_CP041421:20609-20890 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T128382 NZ_CP041421:20609-20890 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGAACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATTCTGCGAGTGTTGCATACAGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT128382 WP_000079941.1 NZ_CP041421:20343-20612 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT128382 NZ_CP041421:20343-20612 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A629TXC5