Detailed information of TA system TA128377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 8794..9419
• Replicon: plasmid pSTEC711_5
• Accession: NZ_CP041421
• Organism: Escherichia coli strain STEC711

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FNE87_RS24775
• Protein ID: WP_000911317.1
• Coordinates: 8794..9192 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: FNE87_RS24780
• Protein ID: WP_000450532.1
• Coordinates: 9192..9419 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE87_RS24760	5120..5617	+	498	WP_000605859.1	entry exclusion protein	-
FNE87_RS24765	5649..6380	+	732	WP_000850430.1	complement resistance protein TraT	-
FNE87_RS24770	6632..8785	+	2154	WP_023568632.1	type IV conjugative transfer system coupling protein TraD	-
FNE87_RS24775	8794..9192	-	399	WP_000911317.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FNE87_RS24780	9192..9419	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..82344	82344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T128377 WP_000911317.1 NZ_CP041421:c9192-8794 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T128377 NZ_CP041421:c9192-8794 [Escherichia coli]
ATGCTGAAATTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGGCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT128377 WP_000450532.1 NZ_CP041421:c9419-9192 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT128377 NZ_CP041421:c9419-9192 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5