Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2603036..2603674 Replicon chromosome
Accession NZ_CP041416
Organism Escherichia coli strain STEC711

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FNE87_RS12545 Protein ID WP_000813794.1
Coordinates 2603498..2603674 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FNE87_RS12540 Protein ID WP_001270286.1
Coordinates 2603036..2603452 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNE87_RS12520 2598188..2599129 - 942 WP_001251315.1 ABC transporter permease -
FNE87_RS12525 2599130..2600143 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FNE87_RS12530 2600161..2601306 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
FNE87_RS12535 2601551..2602957 - 1407 WP_050864085.1 PLP-dependent aminotransferase family protein -
FNE87_RS12540 2603036..2603452 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FNE87_RS12545 2603498..2603674 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FNE87_RS12550 2603896..2604126 + 231 WP_000494244.1 YncJ family protein -
FNE87_RS12555 2604218..2606179 - 1962 WP_050867317.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FNE87_RS12560 2606252..2606788 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FNE87_RS12565 2606841..2608055 + 1215 WP_001348055.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2608095..2609243 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T128366 WP_000813794.1 NZ_CP041416:c2603674-2603498 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T128366 NZ_CP041416:c2603674-2603498 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT128366 WP_001270286.1 NZ_CP041416:c2603452-2603036 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT128366 NZ_CP041416:c2603452-2603036 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References