Detailed information of TA system TA128315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1391134..1391759
• Replicon: chromosome
• Accession: NZ_CP041411
• Organism: Escherichia coli strain STEC719

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FNE88_RS06700
• Protein ID: WP_000911330.1
• Coordinates: 1391361..1391759 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FNE88_RS06695
• Protein ID: WP_000450524.1
• Coordinates: 1391134..1391361 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNE88_RS06670	1386937..1387407	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FNE88_RS06675	1387407..1387979	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FNE88_RS06680	1388125..1389003	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FNE88_RS06685	1389020..1390054	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FNE88_RS06690	1390267..1390980	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FNE88_RS06695	1391134..1391361	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FNE88_RS06700	1391361..1391759	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FNE88_RS06705	1391906..1392769	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
FNE88_RS06710	1392784..1394799	+	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
FNE88_RS06715	1394873..1395571	+	699	WP_000679823.1	esterase	-
FNE88_RS06720	1395681..1395881	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T128315 WP_000911330.1 NZ_CP041411:1391361-1391759 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T128315 NZ_CP041411:1391361-1391759 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT128315 WP_000450524.1 NZ_CP041411:1391134-1391361 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT128315 NZ_CP041411:1391134-1391361 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33