Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 107693..108336 Replicon plasmid p45407_1
Accession NZ_CP041393
Organism Escherichia coli strain ECOL-18-VL-LA-PA-Ryan-0026

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag E3Z24_RS24120 Protein ID WP_001034044.1
Coordinates 107693..108109 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag E3Z24_RS24125 Protein ID WP_001261286.1
Coordinates 108106..108336 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3Z24_RS25450 102946..103059 - 114 Protein_122 IS6 family transposase -
E3Z24_RS24100 103259..103956 + 698 Protein_123 IS1 family transposase -
E3Z24_RS24105 104106..104792 + 687 Protein_124 IS1 family transposase -
E3Z24_RS24110 105046..106068 - 1023 WP_000361402.1 helicase UvrD -
E3Z24_RS24115 106053..107618 - 1566 WP_001128474.1 AAA family ATPase -
E3Z24_RS24120 107693..108109 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
E3Z24_RS24125 108106..108336 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E3Z24_RS24135 108896..109114 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
E3Z24_RS24140 109116..109421 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
E3Z24_RS24145 109422..110228 + 807 WP_000016970.1 site-specific integrase -
E3Z24_RS24150 110950..111705 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aadA5 / qacE / sul1 / blaNDM-5 / dfrA12 / aadA2 / mph(A) - 1..139547 139547


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T128299 WP_001034044.1 NZ_CP041393:c108109-107693 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T128299 NZ_CP041393:c108109-107693 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT128299 WP_001261286.1 NZ_CP041393:c108336-108106 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT128299 NZ_CP041393:c108336-108106 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References