Detailed information of TA system TA128287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2494334..2494972
• Replicon: chromosome
• Accession: NZ_CP041392
• Organism: Escherichia coli strain ECOL-18-VL-LA-PA-Ryan-0026

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: E3Z24_RS11955
• Protein ID: WP_001447010.1
• Coordinates: 2494796..2494972 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E3Z24_RS11950
• Protein ID: WP_001270286.1
• Coordinates: 2494334..2494750 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z24_RS11930	2489486..2490427	-	942	WP_001251304.1	ABC transporter permease	-
E3Z24_RS11935	2490428..2491441	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
E3Z24_RS11940	2491459..2492604	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
E3Z24_RS11945	2492849..2494255	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
E3Z24_RS11950	2494334..2494750	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
E3Z24_RS11955	2494796..2494972	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
E3Z24_RS11960	2495194..2495424	+	231	WP_000494244.1	YncJ family protein	-
E3Z24_RS11965	2495516..2497477	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
E3Z24_RS11970	2497550..2498086	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
E3Z24_RS11975	2498139..2499353	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T128287 WP_001447010.1 NZ_CP041392:c2494972-2494796 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T128287 NZ_CP041392:c2494972-2494796 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT128287 WP_001270286.1 NZ_CP041392:c2494750-2494334 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT128287 NZ_CP041392:c2494750-2494334 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8