Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4207228..4207847 Replicon chromosome
Accession NZ_CP041373
Organism Klebsiella pneumoniae strain KP58

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FM021_RS21170 Protein ID WP_002892050.1
Coordinates 4207629..4207847 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FM021_RS21165 Protein ID WP_002892066.1
Coordinates 4207228..4207602 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FM021_RS21155 4202380..4203573 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FM021_RS21160 4203596..4206742 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
FM021_RS21165 4207228..4207602 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FM021_RS21170 4207629..4207847 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FM021_RS21175 4208006..4208572 + 567 WP_002892030.1 maltose O-acetyltransferase -
FM021_RS30630 4208544..4208684 - 141 WP_004147370.1 hypothetical protein -
FM021_RS21180 4208705..4209175 + 471 WP_002892026.1 YlaC family protein -
FM021_RS21185 4209150..4210601 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
FM021_RS21190 4210702..4211400 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
FM021_RS21195 4211397..4211537 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FM021_RS21200 4211537..4211800 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T128248 WP_002892050.1 NZ_CP041373:4207629-4207847 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T128248 NZ_CP041373:4207629-4207847 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT128248 WP_002892066.1 NZ_CP041373:4207228-4207602 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT128248 NZ_CP041373:4207228-4207602 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References