Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 696804..697438 Replicon chromosome
Accession NZ_CP041372
Organism Bacillus sp. M4U3P1

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag FLK61_RS03955 Protein ID WP_176008232.1
Coordinates 697088..697438 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag FLK61_RS03950 Protein ID WP_176008231.1
Coordinates 696804..697082 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLK61_RS03930 692332..693087 - 756 WP_176011129.1 rhomboid family intramembrane serine protease -
FLK61_RS03935 693192..693545 + 354 WP_176008228.1 holo-ACP synthase -
FLK61_RS03940 693605..694825 + 1221 WP_176008229.1 outer membrane lipoprotein carrier protein LolA -
FLK61_RS03945 695344..696552 + 1209 WP_176008230.1 alanine racemase -
FLK61_RS03950 696804..697082 + 279 WP_176008231.1 antitoxin Antitoxin
FLK61_RS03955 697088..697438 + 351 WP_176008232.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FLK61_RS03960 697597..698424 + 828 WP_176008233.1 STAS domain-containing protein -
FLK61_RS03965 698436..698792 + 357 WP_176008234.1 STAS domain-containing protein -
FLK61_RS03970 698795..699196 + 402 WP_176008235.1 anti-sigma regulatory factor -
FLK61_RS03975 699221..700225 + 1005 WP_176011130.1 PP2C family protein-serine/threonine phosphatase -
FLK61_RS03980 700291..700620 + 330 WP_176008236.1 STAS domain-containing protein -
FLK61_RS03985 700623..701114 + 492 WP_176011131.1 anti-sigma B factor RsbW -
FLK61_RS03990 701077..701880 + 804 WP_176008237.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12903.94 Da        Isoelectric Point: 5.6825

>T128234 WP_176008232.1 NZ_CP041372:697088-697438 [Bacillus sp. M4U3P1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIVQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKQYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDDMMLKVNDALNISIGLIDF

Download         Length: 351 bp

>T128234 NZ_CP041372:697088-697438 [Bacillus sp. M4U3P1]
TTGATAGTCAAACGCGGTGATGTTTATTTTGCAGACTTATCACCAGTCGTTGGGTCAGAGCAGGGAGGAGTAAGACCTGT
TTTGATCGTACAAAACGACATTGGGAACAGATTTAGCCCAACGGTGATTGTTGCAGCAATTACAGCACAAATTCAAAAAG
CAAAACTACCAACACACGTAGAGATCAACGCTAAGCAATACGGATTTGATCGCGACTCGGTCATTTTATTAGAACAAATC
CGCACGATTGACAAACAACGATTAACAGACAAAATTACTCATTTAGATGACGATATGATGTTAAAAGTGAATGACGCATT
GAACATAAGCATTGGCCTTATTGACTTTTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10485.97 Da        Isoelectric Point: 4.2298

>AT128234 WP_176008231.1 NZ_CP041372:696804-697082 [Bacillus sp. M4U3P1]
VSMESTKNITVSLPQQLVNELDALTEGKDEDRCAFIQRATKMYVQEQKKLLIRETMQQGYEEMAKINLMIATESFLVEEE
AESTLDRLVSGV

Download         Length: 279 bp

>AT128234 NZ_CP041372:696804-697082 [Bacillus sp. M4U3P1]
GTGTCTATGGAAAGTACAAAGAACATTACGGTTAGTTTACCGCAACAATTGGTGAATGAATTAGATGCATTAACAGAAGG
TAAAGATGAAGATCGCTGCGCATTTATCCAACGTGCAACAAAAATGTACGTGCAGGAGCAAAAGAAGCTACTCATTCGCG
AGACGATGCAACAGGGGTACGAAGAAATGGCGAAAATCAACTTAATGATTGCAACGGAATCCTTTTTAGTTGAAGAGGAG
GCTGAAAGTACGCTAGATCGCCTTGTTAGTGGGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References