Detailed information of TA system TA128225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4533254..4533882
• Replicon: chromosome
• Accession: NZ_CP041362
• Organism: Citrobacter amalonaticus strain 133355-SW-C4-Cam

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A381GI10
• Locus tag: FEO47_RS21550
• Protein ID: WP_042998865.1
• Coordinates: 4533254..4533640 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FEO47_RS21555
• Protein ID: WP_161215612.1
• Coordinates: 4533640..4533882 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEO47_RS21530	4528607..4529944	+	1338	WP_086512772.1	VWA domain-containing protein	-
FEO47_RS21535	4529962..4531458	+	1497	WP_090050645.1	HAMP domain-containing histidine kinase	-
FEO47_RS21540	4531463..4532149	+	687	WP_054177415.1	response regulator transcription factor	-
FEO47_RS21545	4532294..4533223	+	930	WP_042998866.1	formate dehydrogenase accessory protein FdhE	-
FEO47_RS21550	4533254..4533640	-	387	WP_042998865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FEO47_RS21555	4533640..4533882	-	243	WP_161215612.1	ribbon-helix-helix domain-containing protein	Antitoxin
FEO47_RS21560	4534090..4535016	+	927	WP_090050641.1	alpha/beta hydrolase	-
FEO47_RS21565	4535032..4535973	-	942	WP_042998862.1	fatty acid biosynthesis protein FabY	-
FEO47_RS21570	4536018..4536455	-	438	WP_044265155.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FEO47_RS21575	4536452..4537324	-	873	WP_044257165.1	virulence factor BrkB family protein	-
FEO47_RS21580	4537318..4537917	-	600	WP_044265152.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14404.65 Da
• Isoelectric Point: 9.4114

>T128225 WP_042998865.1 NZ_CP041362:c4533640-4533254 [Citrobacter amalonaticus]
MQKGPVLFDTNILIDLFSGRQEAQQVLEAYPPQNAISLVTWMEVMVGAKKYHQEYRTRVALSAFNIIGVSQEIAERSVNL
RQEYGMKLPDAIILATAQIHRFALVTRNTRDFAGIPGVITPYQLQAGR

Nucleotide

• Length: 387 bp

>T128225 NZ_CP041362:c4533640-4533254 [Citrobacter amalonaticus]
ATGCAAAAGGGTCCCGTGCTGTTCGATACCAATATCCTGATTGATTTATTCAGCGGGCGCCAGGAAGCACAGCAAGTACT
GGAAGCGTATCCGCCGCAGAATGCCATCAGCCTGGTTACCTGGATGGAAGTAATGGTGGGGGCAAAAAAATACCATCAGG
AATATCGTACACGTGTGGCGTTGAGTGCGTTCAACATCATCGGCGTTTCACAGGAAATTGCTGAGCGAAGCGTGAATCTC
AGGCAGGAATATGGGATGAAACTTCCGGACGCGATCATTCTGGCTACCGCGCAGATTCATCGGTTTGCGCTTGTGACGCG
CAACACCAGAGATTTCGCGGGGATACCTGGCGTCATTACGCCTTATCAGTTGCAAGCCGGACGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9335.53 Da
• Isoelectric Point: 4.2075

>AT128225 WP_161215612.1 NZ_CP041362:c4533882-4533640 [Citrobacter amalonaticus]
IMAGMDMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISNALGLWQGCEEDGVEYQRTLREEW

Nucleotide

• Length: 243 bp

>AT128225 NZ_CP041362:c4533882-4533640 [Citrobacter amalonaticus]
ATAATGGCTGGAATGGACATGGGCAGAATATTACTCGACTTATCCGATGAGGTGATCAAGCGCCTTGACGATCTCAAGCA
GCAGCGCAACCTGCCGCGTGCGGAGTTGTTGCGCGAAGCGGTTGAACAGTACCTTGAAAGGCAGGGACAGGCTGAAACCA
CGATTTCCAATGCGCTGGGGCTCTGGCAGGGCTGTGAGGAAGACGGCGTTGAGTATCAGAGGACGCTACGCGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A381GI10