Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3938097..3938673 Replicon chromosome
Accession NZ_CP041362
Organism Citrobacter amalonaticus strain 133355-SW-C4-Cam

Toxin (Protein)


Gene name shpA Uniprot ID A0A8B3ZLZ2
Locus tag FEO47_RS18825 Protein ID WP_044326916.1
Coordinates 3938386..3938673 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A8B3ZM28
Locus tag FEO47_RS18820 Protein ID WP_044326917.1
Coordinates 3938097..3938399 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEO47_RS18800 3933291..3934298 - 1008 WP_044264468.1 Ldh family oxidoreductase -
FEO47_RS18805 3934595..3936745 + 2151 WP_042321961.1 pyruvate/proton symporter BtsT -
FEO47_RS18810 3936853..3937056 + 204 WP_024130742.1 YbdD/YjiX family protein -
FEO47_RS18815 3937069..3938028 + 960 WP_044253881.1 GTPase -
FEO47_RS18820 3938097..3938399 - 303 WP_044326917.1 BrnA antitoxin family protein Antitoxin
FEO47_RS18825 3938386..3938673 - 288 WP_044326916.1 BrnT family toxin Toxin
FEO47_RS18830 3939028..3941370 + 2343 WP_090048357.1 type I restriction-modification system subunit M -
FEO47_RS18835 3941370..3942767 + 1398 WP_090048356.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11095.53 Da        Isoelectric Point: 8.6574

>T128223 WP_044326916.1 NZ_CP041362:c3938673-3938386 [Citrobacter amalonaticus]
MPTEFEWDANKARSNVGKHGVRFEDAILVFDDPRHLSRQDRIENGEYRWQTLGLVHGIVVILVAHSIRFESGIDVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T128223 NZ_CP041362:c3938673-3938386 [Citrobacter amalonaticus]
ATGCCAACGGAGTTTGAATGGGATGCAAATAAGGCAAGAAGCAATGTCGGTAAACATGGTGTCCGTTTTGAAGATGCGAT
TCTGGTATTTGACGATCCACGACATCTTTCACGACAGGATCGCATAGAGAACGGGGAATATCGCTGGCAAACCCTCGGTC
TCGTTCATGGCATCGTAGTCATTCTGGTTGCTCACAGCATTCGGTTTGAAAGTGGTATCGATGTCATTCGCATCATCAGC
GCCAGAAAGGCGGATCGTAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11460.05 Da        Isoelectric Point: 10.1293

>AT128223 WP_044326917.1 NZ_CP041362:c3938399-3938097 [Citrobacter amalonaticus]
MSMVKHKRGAQSSLSAQHEAELKALAKKSDDEIDYSDIPSSPDEQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT128223 NZ_CP041362:c3938399-3938097 [Citrobacter amalonaticus]
ATGAGCATGGTTAAACATAAACGCGGCGCGCAATCCTCGCTCAGTGCTCAGCATGAGGCGGAACTCAAAGCGTTGGCGAA
GAAATCAGATGATGAAATTGATTACAGTGATATCCCCTCTTCGCCAGATGAGCAATGGTCTGAAGCAGTACGCGGTAAGT
TTTTTCGCCCGCTAAAAACCCAGGCGTCCGTACGTATTGACGCGGATGTGATGGAATGGCTGAAGCGTCCGGGGAAAGGG
TATCAGACACGGCTTAACGCGATCTTGCGGGAAGCGATGCTGCGGGAACAAAACAAGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3ZLZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3ZM28

References