Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1272213..1272833 Replicon chromosome
Accession NZ_CP041362
Organism Citrobacter amalonaticus strain 133355-SW-C4-Cam

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag FEO47_RS05970 Protein ID WP_001280991.1
Coordinates 1272213..1272431 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag FEO47_RS05975 Protein ID WP_042324455.1
Coordinates 1272459..1272833 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FEO47_RS05940 1267866..1268462 + 597 WP_142764914.1 hypothetical protein -
FEO47_RS05945 1268545..1268898 + 354 WP_043001561.1 DUF1428 family protein -
FEO47_RS05950 1268938..1270488 - 1551 WP_090048827.1 EAL domain-containing protein -
FEO47_RS05955 1270711..1270851 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
FEO47_RS05960 1270901..1271371 - 471 WP_043001559.1 YlaC family protein -
FEO47_RS05965 1271485..1272036 - 552 WP_044258934.1 maltose O-acetyltransferase -
FEO47_RS05970 1272213..1272431 - 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
FEO47_RS05975 1272459..1272833 - 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
FEO47_RS05980 1273345..1276494 - 3150 WP_142764915.1 efflux RND transporter permease AcrB -
FEO47_RS05985 1276517..1277710 - 1194 WP_043001556.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T128211 WP_001280991.1 NZ_CP041362:c1272431-1272213 [Citrobacter amalonaticus]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T128211 NZ_CP041362:c1272431-1272213 [Citrobacter amalonaticus]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCTTGCGACGCTGTCAGACAATTGACACACTTGAACGCGT
GATTGAGAAAAATAAATATGAATTATCTGACAATGAATTGGCTGTATTTTACTCAGCCGCCGATCACCGTCTTGCAGAAT
TGACGATGAATAAACTTTACGACAAGATCCCCTCCTCCGTCTGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT128211 WP_042324455.1 NZ_CP041362:c1272833-1272459 [Citrobacter amalonaticus]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT128211 NZ_CP041362:c1272833-1272459 [Citrobacter amalonaticus]
ATGGATGAATACTCACCCAAAAGACATGATATAGCGCAGCTTAAATTTCTTTGTGAAACGCTTTATCACGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTTCAACTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGAGCAGATTGATGAATATCTG
GACGACACATTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGCTT
GTTCCGCTGCTTTGTGAATGCCACCAGAGCTAACCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References