Detailed information of TA system TA128208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 510688..511325
• Replicon: chromosome
• Accession: NZ_CP041361
• Organism: Bacillus velezensis strain WRN014

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CXB71_RS02495
• Protein ID: WP_003156187.1
• Coordinates: 510975..511325 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CXB71_RS02490
• Protein ID: WP_003156188.1
• Coordinates: 510688..510969 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXB71_RS02470	507053..507652	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
CXB71_RS02475	507745..508110	+	366	WP_014304402.1	holo-ACP synthase	-
CXB71_RS02480	508275..509282	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
CXB71_RS02485	509399..510568	+	1170	WP_003156189.1	alanine racemase	-
CXB71_RS02490	510688..510969	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CXB71_RS02495	510975..511325	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CXB71_RS02500	511443..512264	+	822	WP_014304404.1	STAS domain-containing protein	-
CXB71_RS02505	512269..512634	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
CXB71_RS02510	512637..513038	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CXB71_RS02515	513050..514057	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
CXB71_RS02520	514121..514450	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CXB71_RS02525	514447..514929	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
CXB71_RS02530	514895..515683	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CXB71_RS02535	515683..516285	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T128208 WP_003156187.1 NZ_CP041361:510975-511325 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T128208 NZ_CP041361:510975-511325 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT128208 WP_003156188.1 NZ_CP041361:510688-510969 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT128208 NZ_CP041361:510688-510969 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5