Detailed information of TA system TA128205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 5621693..5622300
• Replicon: chromosome
• Accession: NZ_CP041360
• Organism: Spirosoma sp. KCTC 42546

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EXU85_RS23125
• Protein ID: WP_142774356.1
• Coordinates: 5621693..5622088 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EXU85_RS23130
• Protein ID: WP_142774357.1
• Coordinates: 5622085..5622300 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXU85_RS23105	5617298..5618053	+	756	WP_111349440.1	hypothetical protein	-
EXU85_RS23110	5618142..5619605	+	1464	WP_142774353.1	tetratricopeptide repeat protein	-
EXU85_RS23115	5619669..5620154	-	486	WP_168207853.1	hypothetical protein	-
EXU85_RS23120	5620424..5621611	-	1188	WP_142774355.1	phosphoglycerate kinase	-
EXU85_RS23125	5621693..5622088	-	396	WP_142774356.1	PIN domain nuclease	Toxin
EXU85_RS23130	5622085..5622300	-	216	WP_142774357.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EXU85_RS23135	5622344..5623534	-	1191	WP_142774358.1	hypothetical protein	-
EXU85_RS23140	5623521..5624498	-	978	WP_142774359.1	DUF2157 domain-containing protein	-
EXU85_RS23145	5624529..5626217	+	1689	WP_142774360.1	VRR-NUC domain-containing protein	-
EXU85_RS23150	5626330..5626731	+	402	WP_142774361.1	DUF1801 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-122)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-45)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15006.53 Da
• Isoelectric Point: 6.4736

>T128205 WP_142774356.1 NZ_CP041360:c5622088-5621693 [Spirosoma sp. KCTC 42546]
MIIDTSVWIDFFRDVQSAQTDLLVELLLAKERLCITPTILQEVLQGVHADNHFERIKTDLLACRMLQIDPVKAAIEAATL
YRTLRKKGVTIRKPNDCLIAHYAIFYDIPVLHNDIDFDQIARFTALRIANA

Nucleotide

• Length: 396 bp

>T128205 NZ_CP041360:c5622088-5621693 [Spirosoma sp. KCTC 42546]
ATGATTATTGATACATCCGTTTGGATAGATTTTTTTAGGGATGTTCAGAGTGCGCAGACAGATTTATTAGTCGAACTCCT
GCTTGCGAAGGAGAGGCTGTGTATTACACCTACGATACTGCAGGAAGTCTTACAGGGAGTACATGCTGATAATCATTTTG
AACGAATAAAAACCGACCTATTAGCCTGTCGCATGTTACAGATTGACCCTGTTAAAGCCGCTATTGAAGCGGCAACGTTA
TACCGAACTCTTCGCAAAAAAGGGGTAACGATTCGTAAACCCAACGATTGTTTGATTGCTCATTACGCCATATTCTACGA
TATTCCTGTTCTGCATAACGATATCGACTTTGACCAAATCGCGCGCTTTACCGCATTACGTATTGCCAACGCGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8403.67 Da
• Isoelectric Point: 5.0477

>AT128205 WP_142774357.1 NZ_CP041360:c5622300-5622085 [Spirosoma sp. KCTC 42546]
MRTNIDINDELLQVAMEISHLKTKKAIVELALQEYINMMRRKDLLSLRGKVQWDGDLDQMRTDNTPTDWDK

Nucleotide

• Length: 216 bp

>AT128205 NZ_CP041360:c5622300-5622085 [Spirosoma sp. KCTC 42546]
ATGAGAACAAATATTGACATCAATGATGAGCTGCTTCAAGTAGCTATGGAAATTAGTCACCTAAAAACAAAAAAAGCGAT
CGTAGAATTGGCTTTGCAGGAGTATATCAACATGATGCGCCGTAAAGATTTGTTGTCTTTACGTGGGAAAGTTCAATGGG
ATGGTGATTTAGACCAGATGCGAACCGATAACACACCAACTGATTGGGATAAATGA