Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2806227..2806845 Replicon chromosome
Accession NZ_CP041359
Organism Escherichia coli strain U1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E3Z32_RS14735 Protein ID WP_001291435.1
Coordinates 2806227..2806445 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E3Z32_RS14740 Protein ID WP_000344800.1
Coordinates 2806471..2806845 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3Z32_RS14700 2801516..2802088 + 573 WP_000779831.1 YbaY family lipoprotein -
E3Z32_RS14705 2802119..2802430 - 312 WP_000409911.1 MGMT family protein -
E3Z32_RS14715 2802809..2803162 + 354 WP_001550741.1 DUF1428 family protein -
E3Z32_RS14720 2803204..2804754 - 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E3Z32_RS14725 2804918..2805388 - 471 WP_000136192.1 YlaC family protein -
E3Z32_RS14730 2805504..2806055 - 552 WP_001550742.1 maltose O-acetyltransferase -
E3Z32_RS14735 2806227..2806445 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E3Z32_RS14740 2806471..2806845 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E3Z32_RS14745 2807391..2810540 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
E3Z32_RS14750 2810563..2811756 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T128187 WP_001291435.1 NZ_CP041359:c2806445-2806227 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T128187 NZ_CP041359:c2806445-2806227 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT128187 WP_000344800.1 NZ_CP041359:c2806845-2806471 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT128187 NZ_CP041359:c2806845-2806471 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References