128184

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2245375..2245883 Replicon chromosome
Accession NZ_CP041359
Organism Escherichia coli strain U1

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag E3Z32_RS12080 Protein ID WP_001534843.1
Coordinates 2245375..2245584 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0V9L4L3
Locus tag E3Z32_RS12085 Protein ID WP_000063161.1
Coordinates 2245581..2245883 (+) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3Z32_RS12065 2241130..2242440 - 1311 WP_001534837.1 restriction endonuclease subunit S -
E3Z32_RS12070 2242430..2244049 - 1620 WP_001550561.1 type I restriction-modification system subunit M -
E3Z32_RS12075 2244113..2245279 - 1167 WP_001534841.1 restriction endonuclease -
E3Z32_RS12080 2245375..2245584 + 210 WP_001534843.1 BrnT family toxin Toxin
E3Z32_RS12085 2245581..2245883 + 303 WP_000063161.1 BrnA antitoxin family protein Antitoxin
E3Z32_RS12090 2245917..2246873 - 957 WP_001534845.1 GTPase -
E3Z32_RS12095 2246884..2247087 - 204 WP_001534846.1 YbdD/YjiX family protein -
E3Z32_RS12100 2247205..2249355 - 2151 WP_001366791.1 pyruvate/proton symporter BtsT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(37-93)


Sequences


Toxin        


Download         Length: 70 a.a.        Molecular weight: 7952.17 Da        Isoelectric Point: 4.9493

>T128184 WP_001534843.1 NZ_CP041359:2245375-2245584 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Download         Length: 210 bp

>T128184 NZ_CP041359:2245375-2245584 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11600.10 Da        Isoelectric Point: 9.4646

>AT128184 WP_000063161.1 NZ_CP041359:2245581-2245883 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT128184 NZ_CP041359:2245581-2245883 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A0V9L4L3

References