Detailed information of TA system TA128180

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1903098..1903933
• Replicon: chromosome
• Accession: NZ_CP041359
• Organism: Escherichia coli strain U1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9IW37
• Locus tag: E3Z32_RS10210
• Protein ID: WP_023356554.1
• Coordinates: 1903098..1903475 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: E3Z32_RS10215
• Protein ID: WP_001360327.1
• Coordinates: 1903565..1903933 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z32_RS10190	1899465..1901003	-	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-
E3Z32_RS10195	1901752..1902594	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
E3Z32_RS27385	1902679..1902873	-	195	WP_024181941.1	DUF957 domain-containing protein	-
E3Z32_RS27390	1902952..1903101	-	150	Protein_1757	hypothetical protein	-
E3Z32_RS10210	1903098..1903475	-	378	WP_023356554.1	TA system toxin CbtA family protein	Toxin
E3Z32_RS10215	1903565..1903933	-	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E3Z32_RS10220	1904096..1904317	-	222	WP_023356553.1	DUF987 domain-containing protein	-
E3Z32_RS10225	1904380..1904856	-	477	WP_023356600.1	RadC family protein	-
E3Z32_RS10230	1904872..1905351	-	480	WP_000706978.1	antirestriction protein	-
E3Z32_RS27225	1905451..1905591	+	141	WP_000997937.1	hypothetical protein	-
E3Z32_RS10235	1905617..1906435	-	819	WP_001175143.1	DUF945 domain-containing protein	-
E3Z32_RS10240	1906525..1906758	-	234	WP_000883181.1	DUF905 family protein	-
E3Z32_RS10245	1906764..1907441	-	678	WP_001097562.1	hypothetical protein	-
E3Z32_RS10250	1907592..1908272	-	681	WP_001278651.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1891976..1945059	53083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14052.01 Da
• Isoelectric Point: 7.8045

>T128180 WP_023356554.1 NZ_CP041359:c1903475-1903098 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFADERVIEQHIEAGIALCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T128180 NZ_CP041359:c1903475-1903098 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT128180 WP_001360327.1 NZ_CP041359:c1903933-1903565 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128180 NZ_CP041359:c1903933-1903565 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9IW37

Antitoxin

• AlphaFold DB: A0A7U9IW59