Detailed information of TA system TA128177

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1320216..1321050
• Replicon: chromosome
• Accession: NZ_CP041359
• Organism: Escherichia coli strain U1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8T5WFL4
• Locus tag: E3Z32_RS07560
• Protein ID: WP_000854823.1
• Coordinates: 1320673..1321050 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8T5WFE1
• Locus tag: E3Z32_RS07555
• Protein ID: WP_001285614.1
• Coordinates: 1320216..1320584 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z32_RS07520	1315936..1316616	+	681	WP_001278651.1	WYL domain-containing protein	-
E3Z32_RS07525	1316767..1317444	+	678	WP_001097562.1	hypothetical protein	-
E3Z32_RS07530	1317450..1317683	+	234	WP_000883181.1	DUF905 family protein	-
E3Z32_RS07535	1317773..1318591	+	819	WP_001175143.1	DUF945 domain-containing protein	-
E3Z32_RS27200	1318617..1318757	-	141	WP_000997937.1	hypothetical protein	-
E3Z32_RS07540	1318857..1319336	+	480	WP_000706978.1	antirestriction protein	-
E3Z32_RS07545	1319352..1319828	+	477	WP_001186792.1	RadC family protein	-
E3Z32_RS07550	1319915..1320136	+	222	WP_000692350.1	DUF987 domain-containing protein	-
E3Z32_RS07555	1320216..1320584	+	369	WP_001285614.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E3Z32_RS07560	1320673..1321050	+	378	WP_000854823.1	TA system toxin CbtA family protein	Toxin
E3Z32_RS07565	1321047..1321196	+	150	Protein_1263	hypothetical protein	-
E3Z32_RS07570	1321272..1321469	+	198	WP_087890155.1	DUF957 domain-containing protein	-
E3Z32_RS07575	1321554..1322396	+	843	WP_001290170.1	DUF4942 domain-containing protein	-
E3Z32_RS27205	1322580..1322762	+	183	WP_177309578.1	hypothetical protein	-
E3Z32_RS07585	1323190..1324374	+	1185	WP_001172876.1	sugar efflux transporter	-
E3Z32_RS07590	1324485..1325408	+	924	WP_000535949.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.04 Da
• Isoelectric Point: 6.7093

>T128177 WP_000854823.1 NZ_CP041359:1320673-1321050 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRMVNDISQGKYPEAKQ

Nucleotide

• Length: 378 bp

>T128177 NZ_CP041359:1320673-1321050 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATGACATTAGCCAGGGGAAGTATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13596.35 Da
• Isoelectric Point: 6.0618

>AT128177 WP_001285614.1 NZ_CP041359:1320216-1320584 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTSCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128177 NZ_CP041359:1320216-1320584 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GTCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA