Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 30398..30933 Replicon plasmid unnamed1
Accession NZ_CP041358
Organism Escherichia coli strain U1

Toxin (Protein)


Gene name relE Uniprot ID A0A0M1UJC2
Locus tag E3Z32_RS00200 Protein ID WP_001270422.1
Coordinates 30398..30685 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag E3Z32_RS00205 Protein ID WP_001132895.1
Coordinates 30682..30933 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3Z32_RS00170 25536..26705 - 1170 WP_001072358.1 hypothetical protein -
E3Z32_RS00175 27072..27260 + 189 WP_001332784.1 hypothetical protein -
E3Z32_RS00180 27381..28121 + 741 WP_001066947.1 tyrosine-type recombinase/integrase -
E3Z32_RS00185 28364..29341 - 978 WP_001309253.1 RepB family plasmid replication initiator protein -
E3Z32_RS00195 29985..30396 - 412 Protein_32 DDE-type integrase/transposase/recombinase -
E3Z32_RS00200 30398..30685 - 288 WP_001270422.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3Z32_RS00205 30682..30933 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
E3Z32_RS00215 31691..32896 + 1206 WP_000817635.1 AAA family ATPase -
E3Z32_RS00220 32893..33855 + 963 WP_000756328.1 ParB/RepB/Spo0J family partition protein -
E3Z32_RS00225 34089..34865 + 777 Protein_37 hypothetical protein -
E3Z32_RS27120 35032..35175 + 144 WP_000270807.1 DUF1281 domain-containing protein -
E3Z32_RS27125 35182..35541 + 360 WP_001336695.1 DUF1281 domain-containing protein -
E3Z32_RS00245 35605..35916 + 312 WP_001309256.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..130003 130003
- flank IS/Tn - - 34512..34652 140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11262.30 Da        Isoelectric Point: 10.4703

>T128162 WP_001270422.1 NZ_CP041358:c30685-30398 [Escherichia coli]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T128162 NZ_CP041358:c30685-30398 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT128162 WP_001132895.1 NZ_CP041358:c30933-30682 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT128162 NZ_CP041358:c30933-30682 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M1UJC2

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References