Detailed information of TA system TA128160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5240851..5241437
• Replicon: chromosome
• Accession: NZ_CP041354
• Organism: Pseudomonas aeruginosa strain AZPAE15042

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: IHMA87_RS25185
• Protein ID: WP_003120987.1
• Coordinates: 5240851..5241150 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IHMA87_RS25190
• Protein ID: WP_003448662.1
• Coordinates: 5241147..5241437 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHMA87_RS25165	5235964..5236497	-	534	WP_031693915.1	type IV pilus biogenesis protein PilP	-
IHMA87_RS25170	5236487..5237812	-	1326	WP_003120992.1	type 4b pilus protein PilO2	-
IHMA87_RS25175	5237816..5239525	-	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
IHMA87_RS25180	5239525..5240649	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
IHMA87_RS25185	5240851..5241150	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IHMA87_RS25190	5241147..5241437	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
IHMA87_RS25195	5241508..5241852	-	345	WP_016851612.1	hypothetical protein	-
IHMA87_RS25200	5241993..5243969	-	1977	WP_031693914.1	DEAD/DEAH box helicase	-
IHMA87_RS25205	5243966..5245855	-	1890	WP_023101908.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5208858..5312808	103950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T128160 WP_003120987.1 NZ_CP041354:5240851-5241150 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T128160 NZ_CP041354:5240851-5241150 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT128160 WP_003448662.1 NZ_CP041354:5241147-5241437 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT128160 NZ_CP041354:5241147-5241437 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73