Detailed information of TA system TA128156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2343486..2344528
• Replicon: chromosome
• Accession: NZ_CP041354
• Organism: Pseudomonas aeruginosa strain AZPAE15042

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: IHMA87_RS11205
• Protein ID: WP_003109777.1
• Coordinates: 2343953..2344528 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: IHMA87_RS11200
• Protein ID: WP_003050245.1
• Coordinates: 2343486..2343956 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHMA87_RS11165	2338878..2340296	-	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-
IHMA87_RS11170	2340286..2341197	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
IHMA87_RS11175	2341194..2341886	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
IHMA87_RS11180	2341883..2342281	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
IHMA87_RS11185	2342293..2342652	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
IHMA87_RS11190	2342669..2342902	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
IHMA87_RS11195	2342899..2343282	-	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
IHMA87_RS11200	2343486..2343956	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
IHMA87_RS11205	2343953..2344528	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
IHMA87_RS11210	2344546..2345460	+	915	WP_003105629.1	AAA family ATPase	-
IHMA87_RS11215	2345457..2345927	+	471	WP_003105626.1	hypothetical protein	-
IHMA87_RS11220	2345924..2346424	+	501	WP_003090159.1	hypothetical protein	-
IHMA87_RS11225	2346424..2347326	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
IHMA87_RS11230	2347365..2348090	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2293665..2389933	96268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T128156 WP_003109777.1 NZ_CP041354:2343953-2344528 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T128156 NZ_CP041354:2343953-2344528 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT128156 WP_003050245.1 NZ_CP041354:2343486-2343956 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT128156 NZ_CP041354:2343486-2343956 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68