Detailed information of TA system TA128138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1771650..1772293
• Replicon: chromosome
• Accession: NZ_CP041353
• Organism: Klebsiella pneumoniae strain RJY9645

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A4GZE5
• Locus tag: FJR44_RS08520
• Protein ID: WP_015874977.1
• Coordinates: 1771650..1772066 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A4GZE4
• Locus tag: FJR44_RS08525
• Protein ID: WP_015874976.1
• Coordinates: 1772063..1772293 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJR44_RS08505	1767053..1768168	-	1116	WP_032447702.1	salmochelin biosynthesis C-glycosyltransferase IroB	-
FJR44_RS08510	1769040..1771214	+	2175	WP_015874979.1	siderophore salmochelin receptor IroN	-
FJR44_RS08515	1771369..1771614	-	246	WP_032447699.1	hypothetical protein	-
FJR44_RS08520	1771650..1772066	-	417	WP_015874977.1	type II toxin-antitoxin system VapC family toxin	Toxin
FJR44_RS08525	1772063..1772293	-	231	WP_015874976.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FJR44_RS08530	1772736..1773116	+	381	WP_015874975.1	YrzE family protein	-
FJR44_RS08535	1773253..1774206	+	954	Protein_1668	IS21 family transposase	-
FJR44_RS08540	1774301..1775027	+	727	Protein_1669	AAA family ATPase	-
FJR44_RS08545	1775071..1775184	-	114	WP_015874970.1	hypothetical protein	-
FJR44_RS08550	1775427..1775636	-	210	WP_032447696.1	TraR/DksA family transcriptional regulator	-
FJR44_RS08555	1775626..1776207	-	582	WP_000937857.1	DUF2857 domain-containing protein	-
FJR44_RS08560	1776192..1776374	-	183	WP_072001695.1	hypothetical protein	-
FJR44_RS08565	1776396..1776581	-	186	WP_000205185.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	iroD / iroC / iroB / iroN / fyuA / ybtE / ybtT / ybtU	1728519..1791969	63450

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14945.39 Da
• Isoelectric Point: 8.5464

>T128138 WP_015874977.1 NZ_CP041353:c1772066-1771650 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAGLRGDRIVVSAVTYAEMRFGATGPKASPHHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T128138 NZ_CP041353:c1772066-1771650 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGGGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCACCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTACCGGGCCTGGTGCTGG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.80 Da
• Isoelectric Point: 4.3533

>AT128138 WP_015874976.1 NZ_CP041353:c1772293-1772063 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMVEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT128138 NZ_CP041353:c1772293-1772063 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGTGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A4GZE5

Antitoxin

• AlphaFold DB: A4GZE4