Detailed information of TA system TA128128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 176992..177545
• Replicon: chromosome
• Accession: NZ_CP041352
• Organism: Casimicrobium huifangae strain SJ-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FKL89_RS00840
• Protein ID: WP_156860853.1
• Coordinates: 177252..177545 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FKL89_RS00835
• Protein ID: WP_156860852.1
• Coordinates: 176992..177264 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FKL89_RS00815	172090..172407	-	318	WP_033990572.1	type II toxin-antitoxin system PrlF family antitoxin	-
FKL89_RS00820	172493..173119	-	627	WP_106685585.1	integrase	-
FKL89_RS00825	173231..176257	-	3027	WP_156860851.1	Tn3 family transposase	-
FKL89_RS00830	176254..176823	-	570	WP_156864497.1	recombinase family protein	-
FKL89_RS00835	176992..177264	+	273	WP_156860852.1	CopG family ribbon-helix-helix protein	Antitoxin
FKL89_RS00840	177252..177545	+	294	WP_156860853.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FKL89_RS20245	177545..177880	+	336	Protein_167	transposase	-
FKL89_RS00850	178378..180219	+	1842	WP_156864499.1	TraI domain-containing protein	-
FKL89_RS00855	180317..181330	-	1014	WP_022580065.1	DUF4917 family protein	-
FKL89_RS00860	181398..182120	-	723	WP_033990568.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	148468..342798	194330
	-	flank	IS/Tn	-	-	176254..176823	569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10799.33 Da
• Isoelectric Point: 5.1583

>T128128 WP_156860853.1 NZ_CP041352:177252-177545 [Casimicrobium huifangae]
VPQVIVTEGAAEGLERCRKFLATKAPDAARRAGQAIERQFLLLETAPDIGRPFPEMPELRELVIAFGDSGYVALYRHEPD
ADAVYILAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T128128 NZ_CP041352:177252-177545 [Casimicrobium huifangae]
GTGCCACAAGTAATCGTCACCGAGGGCGCAGCCGAAGGCCTGGAGCGATGCCGGAAGTTCCTGGCCACCAAGGCCCCGGA
TGCCGCCAGGCGAGCCGGTCAGGCCATCGAACGGCAATTCCTGCTGCTGGAAACGGCTCCCGACATTGGCCGCCCGTTCC
CTGAAATGCCGGAGTTGCGTGAACTGGTTATTGCCTTCGGGGATTCCGGCTATGTAGCCCTGTACCGCCATGAACCTGAC
GCCGATGCGGTCTACATCCTGGCATTCCGGCATCAGAAAGAAGCGGGCTACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10571.84 Da
• Isoelectric Point: 6.9793

>AT128128 WP_156860852.1 NZ_CP041352:176992-177264 [Casimicrobium huifangae]
MATSLKIDETLKGRVHHLAGQRRRSPHWIMLEAIQQYVEREEARESFKQEALASWAAYQETGRHLTGQEVRTWLNTWGTE
DERAVPECHK

Nucleotide

• Length: 273 bp

>AT128128 NZ_CP041352:176992-177264 [Casimicrobium huifangae]
ATGGCAACATCACTCAAAATTGACGAGACGCTGAAAGGCCGCGTCCACCACCTCGCCGGCCAGCGCCGTCGCTCGCCGCA
CTGGATCATGCTGGAAGCCATTCAGCAATACGTCGAGCGCGAGGAAGCCCGCGAGAGCTTCAAGCAGGAAGCCTTGGCTT
CCTGGGCTGCCTATCAGGAAACCGGTCGGCACCTGACTGGCCAGGAAGTGCGCACTTGGTTGAACACCTGGGGCACCGAA
GACGAAAGGGCGGTGCCTGAGTGCCACAAGTAA