Detailed information of TA system TA128122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 223553..224125
• Replicon: chromosome
• Accession: NZ_CP041348
• Organism: Komagataeibacter xylinus strain CGMCC 17276

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FMA36_RS01135
• Protein ID: WP_003618720.1
• Coordinates: 223553..223882 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5HXX4
• Locus tag: FMA36_RS01140
• Protein ID: WP_003618721.1
• Coordinates: 223883..224125 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FMA36_RS01110	219398..219658	+	261	WP_003618686.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
FMA36_RS01115	219655..219948	+	294	WP_003618711.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
FMA36_RS01120	220079..221341	+	1263	WP_159260229.1	sodium:proton antiporter	-
FMA36_RS01125	221341..222024	+	684	WP_003618716.1	DUF1275 domain-containing protein	-
FMA36_RS01130	222279..223352	+	1074	WP_003618718.1	alkene reductase	-
FMA36_RS01135	223553..223882	-	330	WP_003618720.1	endoribonuclease MazF	Toxin
FMA36_RS01140	223883..224125	-	243	WP_003618721.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FMA36_RS01145	224260..224685	-	426	WP_003618781.1	hypothetical protein	-
FMA36_RS01150	224691..226670	-	1980	WP_040510572.1	conjugal transfer protein TraG	-
FMA36_RS01155	226790..227209	+	420	WP_003618779.1	aromatic ring-cleaving dioxygenase	-
FMA36_RS01160	227314..227940	+	627	WP_003618778.1	hypothetical protein	-
FMA36_RS01165	227945..228535	+	591	WP_003618777.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	174150..272209	98059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-107)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12221.05 Da
• Isoelectric Point: 9.2806

>T128122 WP_003618720.1 NZ_CP041348:c223882-223553 [Komagataeibacter xylinus]
MSAWVPDCGDIVWLEFDPQAGREQSGHRPAVVLSPVSYNKKSGMIVCCPTTTKIKGYPFEVPLTGEPTSVVLSDQVRSLD
WRARRAKHKGRVSERELEAVRERTRLLVG

Nucleotide

• Length: 330 bp

>T128122 NZ_CP041348:c223882-223553 [Komagataeibacter xylinus]
ATGTCAGCATGGGTGCCAGATTGCGGAGACATCGTCTGGCTGGAGTTTGATCCTCAGGCAGGGCGTGAACAATCTGGGCA
TCGCCCGGCTGTGGTGCTTTCGCCAGTTAGTTACAACAAAAAATCTGGTATGATTGTTTGTTGCCCGACAACGACAAAAA
TCAAAGGGTATCCCTTTGAAGTGCCTTTGACAGGAGAGCCGACAAGCGTGGTTCTCTCTGATCAGGTTCGCAGTCTTGAT
TGGCGCGCCAGACGAGCAAAGCATAAAGGTCGGGTCTCCGAAAGAGAACTGGAAGCGGTGCGCGAACGAACAAGACTACT
CGTCGGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8730.86 Da
• Isoelectric Point: 4.9810

>AT128122 WP_003618721.1 NZ_CP041348:c224125-223883 [Komagataeibacter xylinus]
MHGTVRKWGNSAAIRLPANILEAVHLQINQAVDVREEDGRIIIQPVHPAVLSLDTLTAAITDDNRHGEVSFGPEIGGEIW

Nucleotide

• Length: 243 bp

>AT128122 NZ_CP041348:c224125-223883 [Komagataeibacter xylinus]
ATGCATGGGACCGTTCGCAAATGGGGGAACAGCGCTGCCATCCGTCTACCAGCCAATATCCTTGAAGCGGTTCACCTACA
GATCAATCAGGCTGTTGATGTCCGGGAAGAAGATGGGCGTATCATCATTCAACCGGTTCACCCTGCTGTCCTTTCGCTGG
ACACCCTGACTGCCGCCATCACGGATGATAACCGTCACGGTGAAGTGAGTTTTGGGCCTGAAATTGGCGGAGAGATCTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6N3T672