Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 40822..41381 Replicon plasmid pSUH-1
Accession NZ_CP041338
Organism Escherichia coli strain CCUG 73778

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag FMA85_RS24820 Protein ID WP_000421272.1
Coordinates 40822..41097 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag FMA85_RS24825 Protein ID WP_001387467.1
Coordinates 41097..41381 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FMA85_RS24790 36872..37612 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
FMA85_RS24795 37733..37915 - 183 WP_042065278.1 hypothetical protein -
FMA85_RS24800 38258..38565 - 308 Protein_37 type II toxin-antitoxin system ParD family antitoxin -
FMA85_RS24805 39058..40227 + 1170 WP_001072355.1 hypothetical protein -
FMA85_RS24810 40260..40388 + 129 Protein_39 IS481 family transposase -
FMA85_RS24815 40423..40716 - 294 WP_001105060.1 hypothetical protein -
FMA85_RS24820 40822..41097 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FMA85_RS24825 41097..41381 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
FMA85_RS24830 41986..42738 + 753 WP_000562172.1 IclR family transcriptional regulator -
FMA85_RS24835 42784..43749 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
FMA85_RS24840 43782..44162 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
FMA85_RS24845 44187..45077 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
FMA85_RS24850 45478..45918 + 441 Protein_47 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / aadA5 / blaTEM-1B - 1..161372 161372


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T128081 WP_000421272.1 NZ_CP041338:c41097-40822 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T128081 NZ_CP041338:c41097-40822 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT128081 WP_001387467.1 NZ_CP041338:c41381-41097 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT128081 NZ_CP041338:c41381-41097 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References