Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 26906..27507 Replicon plasmid pSUH-1
Accession NZ_CP041338
Organism Escherichia coli strain CCUG 73778

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag FMA85_RS24730 Protein ID WP_001216034.1
Coordinates 27127..27507 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag FMA85_RS24725 Protein ID WP_001190712.1
Coordinates 26906..27127 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FMA85_RS24715 23887..25170 - 1284 WP_001553855.1 restriction endonuclease subunit S -
FMA85_RS24720 25167..26723 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
FMA85_RS24725 26906..27127 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FMA85_RS24730 27127..27507 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
FMA85_RS24735 27512..27691 + 180 WP_001513661.1 hypothetical protein -
FMA85_RS24740 27719..28078 + 360 WP_001513660.1 hypothetical protein -
FMA85_RS24745 28002..28415 + 414 Protein_26 DDE-type integrase/transposase/recombinase -
FMA85_RS24750 28365..28682 - 318 WP_001513659.1 hypothetical protein -
FMA85_RS24755 28910..29926 - 1017 WP_012372828.1 IS5-like element IS5 family transposase -
FMA85_RS24760 30134..31537 + 1404 WP_001373486.1 S-methylmethionine permease -
FMA85_RS24765 31524..32456 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / aadA5 / blaTEM-1B - 1..161372 161372


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T128080 WP_001216034.1 NZ_CP041338:27127-27507 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T128080 NZ_CP041338:27127-27507 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT128080 WP_001190712.1 NZ_CP041338:26906-27127 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT128080 NZ_CP041338:26906-27127 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References