Detailed information of TA system TA128073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4091000..4091800
• Replicon: chromosome
• Accession: NZ_CP041337
• Organism: Escherichia coli strain CCUG 73778

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: FMA85_RS19830
• Protein ID: WP_000342452.1
• Coordinates: 4091273..4091800 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: FMA85_RS19825
• Protein ID: WP_001277107.1
• Coordinates: 4091000..4091266 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FMA85_RS19805	4086659..4087327	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
FMA85_RS19810	4087320..4088378	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
FMA85_RS19815	4088623..4089477	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
FMA85_RS19820	4089748..4090851	+	1104	WP_074171252.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
FMA85_RS19825	4091000..4091266	+	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
FMA85_RS19830	4091273..4091800	+	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
FMA85_RS19835	4091797..4092180	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
FMA85_RS19840	4092603..4093712	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
FMA85_RS19845	4093760..4094686	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
FMA85_RS19850	4094683..4095960	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
FMA85_RS19855	4095957..4096724	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T128073 WP_000342452.1 NZ_CP041337:4091273-4091800 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T128073 NZ_CP041337:4091273-4091800 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT128073 WP_001277107.1 NZ_CP041337:4091000-4091266 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT128073 NZ_CP041337:4091000-4091266 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57