Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 670018..670849 Replicon chromosome
Accession NZ_CP041337
Organism Escherichia coli strain CCUG 73778

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag FMA85_RS03050 Protein ID WP_000854815.1
Coordinates 670018..670392 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag FMA85_RS03055 Protein ID WP_001280918.1
Coordinates 670481..670849 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FMA85_RS03010 665414..666580 + 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
FMA85_RS03015 666699..667172 + 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
FMA85_RS03020 667370..668428 + 1059 WP_001200889.1 FUSC family protein -
FMA85_RS03025 668600..668929 + 330 WP_000450409.1 DUF496 family protein -
FMA85_RS03030 669030..669212 - 183 WP_001296208.1 hypothetical protein -
FMA85_RS03035 669266..669412 + 147 Protein_595 transposase domain-containing protein -
FMA85_RS03040 669701..669814 - 114 WP_001161660.1 DUF957 domain-containing protein -
FMA85_RS03045 669827..670021 - 195 WP_000988600.1 hypothetical protein -
FMA85_RS03050 670018..670392 - 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
FMA85_RS03055 670481..670849 - 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FMA85_RS03060 670865..671509 - 645 WP_000086752.1 hypothetical protein -
FMA85_RS03065 671528..671749 - 222 WP_000692345.1 DUF987 domain-containing protein -
FMA85_RS03070 671812..672288 - 477 WP_001186200.1 RadC family protein -
FMA85_RS03075 672304..672777 - 474 WP_001542276.1 antirestriction protein -
FMA85_RS03080 672871..673116 - 246 WP_001164966.1 hypothetical protein -
FMA85_RS03085 673116..673934 - 819 WP_001542275.1 DUF945 domain-containing protein -
FMA85_RS03090 674155..674565 - 411 WP_000846703.1 hypothetical protein -
FMA85_RS03095 674581..674931 - 351 Protein_607 hypothetical protein -
FMA85_RS03100 675014..675760 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T128053 WP_000854815.1 NZ_CP041337:c670392-670018 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T128053 NZ_CP041337:c670392-670018 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT128053 WP_001280918.1 NZ_CP041337:c670849-670481 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT128053 NZ_CP041337:c670849-670481 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References