128045

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 588439..589019 Replicon chromosome
Accession NZ_CP041334
Organism Glaesserella parasuis strain HPS412

Toxin (Protein)


Gene name mazF Uniprot ID A0A836MFL2
Locus tag FLK62_RS02905 Protein ID WP_021114594.1
Coordinates 588675..589019 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U4SAI4
Locus tag FLK62_RS02900 Protein ID WP_005710601.1
Coordinates 588439..588678 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLK62_RS02875 584143..584835 - 693 WP_010785974.1 tellurite resistance TerB family protein -
FLK62_RS02880 584897..585745 - 849 WP_149350759.1 ABC transporter permease subunit -
FLK62_RS02885 585742..586527 - 786 WP_176443467.1 ABC transporter permease subunit -
FLK62_RS02890 586517..587269 - 753 WP_102033221.1 phosphonate ABC transporter ATP-binding protein -
FLK62_RS02895 587378..588253 - 876 WP_176443468.1 putative selenate ABC transporter substrate-binding protein -
FLK62_RS02900 588439..588678 + 240 WP_005710601.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FLK62_RS02905 588675..589019 + 345 WP_021114594.1 endoribonuclease MazF Toxin
FLK62_RS02910 589025..589693 + 669 WP_176443469.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
FLK62_RS02915 589706..591097 + 1392 WP_160433564.1 MATE family efflux transporter -
FLK62_RS02920 591170..593275 - 2106 WP_176443470.1 hypothetical protein -
FLK62_RS02925 593481..593777 - 297 WP_005710591.1 DUF5374 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12801.77 Da        Isoelectric Point: 7.3830

>T128045 WP_021114594.1 NZ_CP041334:588675-589019 [Glaesserella parasuis]
MTTQEYIPDVGDIIWLDFDPQAGHEQAGHRPALVLTPAIYNRQTGLLICCPLTTKVKGYPFEVDIAGTPQNVVLSDQIKS
LDWRIRHAKFKGKINHHQLSEVKAKIATLLQISE

Download         Length: 345 bp

>T128045 NZ_CP041334:588675-589019 [Glaesserella parasuis]
ATGACTACTCAAGAGTATATCCCTGACGTTGGTGATATTATTTGGCTTGATTTTGATCCGCAAGCAGGACATGAACAAGC
TGGTCATCGTCCAGCATTAGTGTTAACGCCCGCGATTTATAATCGTCAAACAGGGTTGTTAATTTGCTGCCCGCTCACAA
CAAAAGTAAAAGGCTATCCTTTTGAAGTCGATATTGCAGGTACCCCTCAAAATGTTGTACTTTCCGATCAAATTAAAAGC
CTTGACTGGCGTATTCGTCACGCTAAATTTAAAGGTAAAATTAACCATCATCAGTTAAGCGAAGTAAAAGCTAAAATTGC
GACTTTGCTACAAATCAGTGAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8782.21 Da        Isoelectric Point: 5.0351

>AT128045 WP_005710601.1 NZ_CP041334:588439-588678 [Glaesserella parasuis]
MQVAVKKWGNSVGIRIPALLSNSLKLQAESLVDIQEVDGRLIIEPIKPKYNLEQLLSGITEDNLHNEVDFGKPVGKEML

Download         Length: 240 bp

>AT128045 NZ_CP041334:588439-588678 [Glaesserella parasuis]
ATGCAAGTGGCTGTCAAAAAATGGGGAAATAGCGTAGGCATTCGCATTCCTGCTTTATTATCTAATTCATTGAAATTACA
GGCAGAGAGTTTAGTCGATATTCAAGAAGTTGATGGTCGCCTAATTATTGAGCCGATTAAACCTAAATATAATTTAGAGC
AACTTCTTTCGGGCATTACTGAAGATAATTTGCACAATGAAGTCGATTTTGGCAAGCCTGTTGGCAAGGAAATGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836MFL2

Antitoxin

Source ID Structure
AlphaFold DB U4SAI4

References