Detailed information of TA system TA128043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 1389772..1390408
• Replicon: chromosome
• Accession: NZ_CP041305
• Organism: Cytobacillus ciccensis strain 5L6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W7KPR3
• Locus tag: FI644_RS06990
• Protein ID: WP_009336311.1
• Coordinates: 1389772..1390122 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: FI644_RS06995
• Protein ID: WP_053477859.1
• Coordinates: 1390127..1390408 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FI644_RS06965	1385138..1385251	+	114	WP_142108194.1	cortex morphogenetic protein CmpA	-
FI644_RS06970	1385641..1387779	-	2139	WP_181758211.1	RNA-binding transcriptional accessory protein	-
FI644_RS06975	1387956..1388741	-	786	WP_142108196.1	RNA polymerase sigma factor SigB	-
FI644_RS06980	1388719..1389192	-	474	WP_142108197.1	anti-sigma B factor RsbW	-
FI644_RS06985	1389189..1389521	-	333	WP_056682040.1	anti-sigma factor antagonist	-
FI644_RS06990	1389772..1390122	-	351	WP_009336311.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FI644_RS06995	1390127..1390408	-	282	WP_053477859.1	CopG family ribbon-helix-helix protein	Antitoxin
FI644_RS07000	1390587..1391756	-	1170	WP_181758212.1	alanine racemase	-
FI644_RS07005	1391882..1392889	-	1008	WP_053477861.1	outer membrane lipoprotein carrier protein LolA	-
FI644_RS07010	1393099..1393467	-	369	WP_053477862.1	holo-ACP synthase	-
FI644_RS07015	1393726..1394454	+	729	WP_053477863.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13006.03 Da
• Isoelectric Point: 4.8998

>T128043 WP_009336311.1 NZ_CP041305:c1390122-1389772 [Cytobacillus ciccensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEAVQISLGLIEF

Nucleotide

• Length: 351 bp

>T128043 NZ_CP041305:c1390122-1389772 [Cytobacillus ciccensis]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGTTCAGAACAAGGTGGCGTTCGTCCAGT
GCTTGTCATCCAAAACGATATCGGGAATCGGTTTAGTCCCACAGTAATTGTTGCAGCAATCACTGCTCAAATTCAAAAAG
CTAAGCTGCCTACTCATGTTGAAATTGATGCGAAGCGTTATGGCTTTGAACGGGATTCGGTTATTCTTTTAGAACAAATT
CGTACAATTGATAAACAGCGCTTAACCGATAAGATTACCCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCTGT
GCAGATTAGCCTTGGTCTCATCGAATTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10769.30 Da
• Isoelectric Point: 7.2038

>AT128043 WP_053477859.1 NZ_CP041305:c1390408-1390127 [Cytobacillus ciccensis]
VAESSATTEIMVKLPQHLLTELDGFAKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEF
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT128043 NZ_CP041305:c1390408-1390127 [Cytobacillus ciccensis]
GTGGCTGAGTCTAGCGCAACAACAGAGATAATGGTTAAATTACCGCAACATCTATTAACAGAGTTAGATGGTTTTGCTAA
ACAGGAAAACGTTAACCGGAGTGAATTCATTTATCAAGCAACCAAAATGTATTTGCGTGAACGGAAAAAGAGACAAATTC
GTGAATCCATGCGTCGTGGCTATATGGAGATGGCGAAAATAAATTTGACGATTGCATCTGAAGCATTTCAAGCGGAATTC
GAGGCAGAACATACAGTTGAACGTCTAGTAAGCGGAGGGTAA

Structures

Toxin

• AlphaFold DB: A0A169FD46