Detailed information of TA system TA128039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4034698..4035122
• Replicon: chromosome
• Accession: NZ_CP041304
• Organism: Escherichia coli strain MSHS 133

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: FK544_RS25715
• Protein ID: WP_001306201.1
• Coordinates: 4034698..4034886 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0J2BBI0
• Locus tag: FK544_RS20985
• Protein ID: WP_000125563.1
• Coordinates: 4034889..4035122 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK544_RS20955	4030260..4031546	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
FK544_RS20960	4031546..4032535	+	990	WP_000241249.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
FK544_RS20965	4032532..4033353	+	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
FK544_RS20970	4033356..4033733	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
FK544_RS20975	4033740..4034588	+	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
FK544_RS25710	4034585..4034701	-	117	WP_000148634.1	CcdB family protein	-
FK544_RS25715	4034698..4034886	-	189	WP_001306201.1	CcdB family protein	Toxin
FK544_RS20985	4034889..4035122	-	234	WP_000125563.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
FK544_RS20990	4035208..4035687	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
FK544_RS20995	4035879..4037741	-	1863	WP_000377115.1	glutathione-regulated potassium-efflux system protein KefC	-
FK544_RS21000	4037734..4038264	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
FK544_RS21005	4038372..4039703	-	1332	WP_001183198.1	MFS transporter	-
FK544_RS21010	4039762..4040049	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7167.44 Da
• Isoelectric Point: 11.6113

>T128039 WP_001306201.1 NZ_CP041304:c4034886-4034698 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T128039 NZ_CP041304:c4034886-4034698 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8542.58 Da
• Isoelectric Point: 5.8062

>AT128039 WP_000125563.1 NZ_CP041304:c4035122-4034889 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAAIRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT128039 NZ_CP041304:c4035122-4034889 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAATACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0J2BBI0