Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3844339..3845137 Replicon chromosome
Accession NZ_CP041304
Organism Escherichia coli strain MSHS 133

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag FK544_RS20105 Protein ID WP_000854730.1
Coordinates 3844760..3845137 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag FK544_RS20100 Protein ID WP_001285481.1
Coordinates 3844339..3844713 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK544_RS20060 3840314..3840766 + 453 WP_000682723.1 hypothetical protein -
FK544_RS20065 3840884..3841117 + 234 WP_001213776.1 DUF905 family protein -
FK544_RS20070 3841217..3842038 + 822 WP_001234359.1 DUF945 domain-containing protein -
FK544_RS20075 3842038..3842283 + 246 WP_001164966.1 hypothetical protein -
FK544_RS20080 3842377..3842850 + 474 WP_001313575.1 antirestriction protein -
FK544_RS20085 3842866..3843342 + 477 WP_001313574.1 RadC family protein -
FK544_RS20090 3843405..3843626 + 222 WP_000692315.1 DUF987 domain-containing protein -
FK544_RS20095 3843645..3844289 + 645 WP_000086759.1 hypothetical protein -
FK544_RS20100 3844339..3844713 + 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FK544_RS20105 3844760..3845137 + 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
FK544_RS20110 3845134..3845626 + 493 Protein_3672 hypothetical protein -
FK544_RS20115 3845705..3846693 - 989 Protein_3673 IS630 family transposase -
FK544_RS20120 3846841..3848434 - 1594 Protein_3674 IS66 family transposase -
FK544_RS20125 3848465..3848815 - 351 WP_000624707.1 IS66 family insertion sequence element accessory protein TnpB -
FK544_RS20130 3848812..3849103 - 292 Protein_3676 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3825365..3853379 28014


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T128038 WP_000854730.1 NZ_CP041304:3844760-3845137 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T128038 NZ_CP041304:3844760-3845137 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT128038 WP_001285481.1 NZ_CP041304:3844339-3844713 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT128038 NZ_CP041304:3844339-3844713 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References