Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3596090..3596708 Replicon chromosome
Accession NZ_CP041304
Organism Escherichia coli strain MSHS 133

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FK544_RS18885 Protein ID WP_001291435.1
Coordinates 3596490..3596708 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A8S7S0Y3
Locus tag FK544_RS18880 Protein ID WP_039004128.1
Coordinates 3596090..3596464 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK544_RS18870 3591179..3592372 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FK544_RS18875 3592395..3595544 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FK544_RS18880 3596090..3596464 + 375 WP_039004128.1 Hha toxicity modulator TomB Antitoxin
FK544_RS18885 3596490..3596708 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FK544_RS18890 3596880..3597431 + 552 WP_000102564.1 maltose O-acetyltransferase -
FK544_RS18895 3597547..3598017 + 471 WP_000136192.1 YlaC family protein -
FK544_RS18900 3598181..3599731 + 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FK544_RS18905 3599773..3600126 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FK544_RS18915 3600505..3600816 + 312 WP_000409911.1 MGMT family protein -
FK544_RS18920 3600847..3601419 - 573 WP_000779821.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T128037 WP_001291435.1 NZ_CP041304:3596490-3596708 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T128037 NZ_CP041304:3596490-3596708 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14587.42 Da        Isoelectric Point: 4.7395

>AT128037 WP_039004128.1 NZ_CP041304:3596090-3596464 [Escherichia coli]
MDEYSPKRHDITQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT128037 NZ_CP041304:3596090-3596464 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCACGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure

References