Detailed information of TA system TA128035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3175993..3176698
• Replicon: chromosome
• Accession: NZ_CP041304
• Organism: Escherichia coli strain MSHS 133

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: FK544_RS16645
• Protein ID: WP_000539521.1
• Coordinates: 3175993..3176379 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FK544_RS16650
• Protein ID: WP_001280945.1
• Coordinates: 3176369..3176698 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK544_RS16625	3171997..3172623	+	627	WP_001295292.1	glutathione S-transferase GstB	-
FK544_RS16630	3172620..3173735	-	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
FK544_RS16635	3173846..3174229	-	384	WP_000497137.1	biofilm formation regulator BssR	-
FK544_RS16640	3174442..3175767	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
FK544_RS16645	3175993..3176379	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FK544_RS16650	3176369..3176698	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
FK544_RS16655	3176768..3178096	-	1329	WP_000086919.1	GGDEF domain-containing protein	-
FK544_RS16660	3178104..3179543	-	1440	WP_021525609.1	EAL domain-containing protein	-
FK544_RS16665	3179640..3180453	-	814	Protein_3020	CHASE9 sensor domain-containing protein	-
FK544_RS16670	3180631..3181542	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T128035 WP_000539521.1 NZ_CP041304:3175993-3176379 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T128035 NZ_CP041304:3175993-3176379 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT128035 WP_001280945.1 NZ_CP041304:3176369-3176698 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT128035 NZ_CP041304:3176369-3176698 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6