Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2603712..2604348 Replicon chromosome
Accession NZ_CP041304
Organism Escherichia coli strain MSHS 133

Toxin (Protein)


Gene name hicA Uniprot ID A0A5F1EUB3
Locus tag FK544_RS13790 Protein ID WP_000813796.1
Coordinates 2604172..2604348 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FK544_RS13785 Protein ID WP_076838470.1
Coordinates 2603712..2604128 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK544_RS13765 2598864..2599805 - 942 WP_001251307.1 ABC transporter permease -
FK544_RS13770 2599806..2600819 - 1014 WP_000220433.1 ABC transporter ATP-binding protein -
FK544_RS13775 2600837..2601982 - 1146 WP_000047448.1 ABC transporter substrate-binding protein -
FK544_RS13780 2602227..2603633 - 1407 WP_000760605.1 PLP-dependent aminotransferase family protein -
FK544_RS13785 2603712..2604128 - 417 WP_076838470.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FK544_RS13790 2604172..2604348 - 177 WP_000813796.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FK544_RS13795 2604570..2604800 + 231 WP_000494243.1 YncJ family protein -
FK544_RS13800 2604892..2606853 - 1962 WP_001317809.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FK544_RS13805 2606926..2607462 - 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
FK544_RS13810 2607515..2608726 + 1212 WP_071593090.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6751.78 Da        Isoelectric Point: 11.5666

>T128033 WP_000813796.1 NZ_CP041304:c2604348-2604172 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Download         Length: 177 bp

>T128033 NZ_CP041304:c2604348-2604172 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15219.59 Da        Isoelectric Point: 4.5908

>AT128033 WP_076838470.1 NZ_CP041304:c2604128-2603712 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT128033 NZ_CP041304:c2604128-2603712 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5F1EUB3

Antitoxin

Source ID Structure

References