Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1392791..1393416 Replicon chromosome
Accession NZ_CP041304
Organism Escherichia coli strain MSHS 133

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag FK544_RS07745 Protein ID WP_000911329.1
Coordinates 1393018..1393416 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag FK544_RS07740 Protein ID WP_000450524.1
Coordinates 1392791..1393018 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK544_RS07715 1388591..1389061 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
FK544_RS07720 1389061..1389633 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
FK544_RS07725 1389779..1390657 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
FK544_RS07730 1390674..1391708 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
FK544_RS07735 1391921..1392634 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
FK544_RS07740 1392791..1393018 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
FK544_RS07745 1393018..1393416 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
FK544_RS07750 1393563..1394426 + 864 WP_001267498.1 neutral zinc metallopeptidase -
FK544_RS07755 1394441..1396456 + 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
FK544_RS07760 1396530..1397228 + 699 WP_000679819.1 esterase -
FK544_RS07765 1397309..1397509 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T128023 WP_000911329.1 NZ_CP041304:1393018-1393416 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T128023 NZ_CP041304:1393018-1393416 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT128023 WP_000450524.1 NZ_CP041304:1392791-1393018 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT128023 NZ_CP041304:1392791-1393018 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References