Detailed information of TA system TA128019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 352321..352952
• Replicon: chromosome
• Accession: NZ_CP041304
• Organism: Escherichia coli strain MSHS 133

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: FK544_RS02720
• Protein ID: WP_001260301.1
• Coordinates: 352321..352596 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: FK544_RS02725
• Protein ID: WP_000593557.1
• Coordinates: 352593..352952 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK544_RS02705	347325..348392	+	1068	WP_000361470.1	HlyD family secretion protein	-
FK544_RS02710	348389..351124	+	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
FK544_RS02715	351124..352248	+	1125	WP_001318088.1	ABC transporter permease	-
FK544_RS02720	352321..352596	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
FK544_RS02725	352593..352952	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FK544_RS02730	353072..353473	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
FK544_RS02735	353479..354285	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
FK544_RS02740	354282..355046	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
FK544_RS02745	355046..355879	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
FK544_RS02750	355876..356820	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	348389..385434	37045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T128019 WP_001260301.1 NZ_CP041304:352321-352596 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T128019 NZ_CP041304:352321-352596 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT128019 WP_000593557.1 NZ_CP041304:352593-352952 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT128019 NZ_CP041304:352593-352952 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1