Detailed information of TA system TA128015

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 44285..45120
• Replicon: chromosome
• Accession: NZ_CP041304
• Organism: Escherichia coli strain MSHS 133

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TIK8
• Locus tag: FK544_RS01235
• Protein ID: WP_000854694.1
• Coordinates: 44285..44662 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8S7S1S5
• Locus tag: FK544_RS01240
• Protein ID: WP_065793167.1
• Coordinates: 44752..45120 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK544_RS01195	39419..39712	-	294	WP_001297978.1	hypothetical protein	-
FK544_RS01200	39934..40752	+	819	WP_000779405.1	lipoprotein NlpA	-
FK544_RS01205	40756..41679	-	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
FK544_RS01210	41846..42343	-	498	WP_000509808.1	hypothetical protein	-
FK544_RS01220	42939..43781	-	843	WP_001696593.1	DUF4942 domain-containing protein	-
FK544_RS01225	43866..44063	-	198	WP_086795284.1	DUF957 domain-containing protein	-
FK544_RS01230	44139..44288	-	150	Protein_44	hypothetical protein	-
FK544_RS01235	44285..44662	-	378	WP_000854694.1	TA system toxin CbtA family protein	Toxin
FK544_RS01240	44752..45120	-	369	WP_065793167.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FK544_RS01245	45283..45504	-	222	WP_000692300.1	DUF987 domain-containing protein	-
FK544_RS01250	45567..46043	-	477	WP_001186710.1	RadC family protein	-
FK544_RS01255	46055..46534	-	480	WP_000860065.1	antirestriction protein	-
FK544_RS01260	46616..47434	-	819	WP_001175149.1	DUF945 domain-containing protein	-
FK544_RS01265	47524..47757	-	234	WP_001278287.1	DUF905 family protein	-
FK544_RS01270	47763..48440	-	678	WP_001097305.1	hypothetical protein	-
FK544_RS01275	48588..49268	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14008.02 Da
• Isoelectric Point: 7.7293

>T128015 WP_000854694.1 NZ_CP041304:c44662-44285 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T128015 NZ_CP041304:c44662-44285 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13555.50 Da
• Isoelectric Point: 6.6249

>AT128015 WP_065793167.1 NZ_CP041304:c45120-44752 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128015 NZ_CP041304:c45120-44752 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2F934