Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 118760..119295 | Replicon | plasmid pCys-11 |
| Accession | NZ_CP041303 | ||
| Organism | Escherichia coli strain MSHS 133 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | A0A2H5C964 |
| Locus tag | FK544_RS00760 | Protein ID | WP_000222774.1 |
| Coordinates | 118760..119047 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0VIP2 |
| Locus tag | FK544_RS00765 | Protein ID | WP_001132900.1 |
| Coordinates | 119044..119295 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FK544_RS00720 | 114732..115183 | - | 452 | Protein_124 | N-acetyltransferase | - |
| FK544_RS00725 | 115511..115771 | + | 261 | WP_000371881.1 | hypothetical protein | - |
| FK544_RS00730 | 115768..116358 | + | 591 | WP_021531027.1 | hypothetical protein | - |
| FK544_RS00735 | 116378..116716 | - | 339 | WP_000142460.1 | hypothetical protein | - |
| FK544_RS00740 | 116845..117180 | + | 336 | WP_021531026.1 | immunity protein | - |
| FK544_RS00745 | 117202..117990 | - | 789 | Protein_129 | hypothetical protein | - |
| FK544_RS00755 | 118186..118590 | - | 405 | WP_000154256.1 | DUF2251 domain-containing protein | - |
| FK544_RS00760 | 118760..119047 | - | 288 | WP_000222774.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| FK544_RS00765 | 119044..119295 | - | 252 | WP_001132900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| FK544_RS25535 | 119419..119559 | - | 141 | WP_161953058.1 | hypothetical protein | - |
| FK544_RS00775 | 120259..121116 | - | 858 | WP_000774296.1 | incFII family plasmid replication initiator RepA | - |
| FK544_RS00780 | 121109..121591 | - | 483 | WP_001273588.1 | hypothetical protein | - |
| FK544_RS00785 | 121584..121658 | - | 75 | WP_001442103.1 | RepA leader peptide Tap | - |
| FK544_RS00795 | 121890..122147 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| FK544_RS00800 | 122431..122637 | - | 207 | WP_001351576.1 | type I toxin-antitoxin system Hok family toxin | - |
| - | 122624..122685 | + | 62 | NuclAT_1 | - | - |
| - | 122624..122685 | + | 62 | NuclAT_1 | - | - |
| - | 122624..122685 | + | 62 | NuclAT_1 | - | - |
| - | 122624..122685 | + | 62 | NuclAT_1 | - | - |
| FK544_RS25630 | 122941..123015 | - | 75 | Protein_139 | endonuclease | - |
| FK544_RS00810 | 123261..123473 | - | 213 | WP_001299730.1 | ANR family transcriptional regulator | - |
| FK544_RS00815 | 123609..124169 | - | 561 | WP_000139315.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sitABCD | iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroD / iroE / iroN | 1..157660 | 157660 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11133.20 Da Isoelectric Point: 10.5832
>T128009 WP_000222774.1 NZ_CP041303:c119047-118760 [Escherichia coli]
MTYTVKFRDDALKEWVKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
MTYTVKFRDDALKEWVKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
Download Length: 288 bp
>T128009 NZ_CP041303:c119047-118760 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGGTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGGTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG
Antitoxin
Download Length: 84 a.a. Molecular weight: 9211.41 Da Isoelectric Point: 4.0780
>AT128009 WP_001132900.1 NZ_CP041303:c119295-119044 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT128009 NZ_CP041303:c119295-119044 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2H5C964 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0VIP2 |