Detailed information of TA system TA128008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 101620..102167
• Replicon: plasmid pCys-11
• Accession: NZ_CP041303
• Organism: Escherichia coli strain MSHS 133

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: FK544_RS00630
• Protein ID: WP_000969989.1
• Coordinates: 101620..101901 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: FK544_RS00635
• Protein ID: WP_000079941.1
• Coordinates: 101898..102167 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK544_RS00605	98625..98885	+	261	WP_000371881.1	hypothetical protein	-
FK544_RS00610	98882..99472	+	591	WP_001376212.1	hypothetical protein	-
FK544_RS00615	99490..99837	-	348	WP_000142441.1	hypothetical protein	-
FK544_RS00620	100127..100609	-	483	WP_001311056.1	hypothetical protein	-
FK544_RS00625	100726..101574	-	849	WP_001058004.1	3'-5' exonuclease	-
FK544_RS00630	101620..101901	-	282	WP_000969989.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FK544_RS00635	101898..102167	-	270	WP_000079941.1	hypothetical protein	Antitoxin
FK544_RS00640	102778..104052	+	1275	WP_000489607.1	HlyD family secretion protein	-
FK544_RS00645	104027..106141	+	2115	WP_001183605.1	peptidase domain-containing ABC transporter	-
FK544_RS00650	106311..106622	-	312	WP_001259758.1	colicin V	-
FK544_RS00655	106600..106836	-	237	WP_014640552.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroD / iroE / iroN	1..157660	157660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10902.56 Da
• Isoelectric Point: 8.4615

>T128008 WP_000969989.1 NZ_CP041303:c101901-101620 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T128008 NZ_CP041303:c101901-101620 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTAGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT128008 WP_000079941.1 NZ_CP041303:c102167-101898 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT128008 NZ_CP041303:c102167-101898 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5