Detailed information of TA system TA128007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 95329..95972
• Replicon: plasmid pCys-11
• Accession: NZ_CP041303
• Organism: Escherichia coli strain MSHS 133

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: FK544_RS00595
• Protein ID: WP_001044766.1
• Coordinates: 95556..95972 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: FK544_RS00590
• Protein ID: WP_001261287.1
• Coordinates: 95329..95559 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK544_RS00555	90601..91209	-	609	WP_000715518.1	hypothetical protein	-
FK544_RS00560	91279..91551	-	273	Protein_93	hypothetical protein	-
FK544_RS00565	91707..92633	+	927	WP_001280608.1	hypothetical protein	-
FK544_RS00570	92677..92931	+	255	WP_000678529.1	hypothetical protein	-
FK544_RS00575	93428..93772	+	345	WP_000793772.1	hypothetical protein	-
FK544_RS25625	93803..93943	+	141	WP_154071810.1	hypothetical protein	-
FK544_RS00580	93956..94957	-	1002	WP_000138754.1	DUF4238 domain-containing protein	-
FK544_RS00590	95329..95559	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FK544_RS00595	95556..95972	+	417	WP_001044766.1	type II toxin-antitoxin system VapC family toxin	Toxin
FK544_RS00600	96134..98272	-	2139	WP_000350635.1	AAA family ATPase	-
FK544_RS00605	98625..98885	+	261	WP_000371881.1	hypothetical protein	-
FK544_RS00610	98882..99472	+	591	WP_001376212.1	hypothetical protein	-
FK544_RS00615	99490..99837	-	348	WP_000142441.1	hypothetical protein	-
FK544_RS00620	100127..100609	-	483	WP_001311056.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroD / iroE / iroN	1..157660	157660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15241.71 Da
• Isoelectric Point: 7.7805

>T128007 WP_001044766.1 NZ_CP041303:95556-95972 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AIDATTKIKVALRLTGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T128007 NZ_CP041303:95556-95972 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAATAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGACCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT128007 WP_001261287.1 NZ_CP041303:95329-95559 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT128007 NZ_CP041303:95329-95559 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QFC4