Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2476356..2476994 Replicon chromosome
Accession NZ_CP041302
Organism Escherichia coli strain MSHS 472

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FK543_RS13080 Protein ID WP_000813794.1
Coordinates 2476818..2476994 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FK543_RS13075 Protein ID WP_001270286.1
Coordinates 2476356..2476772 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK543_RS13055 2471508..2472449 - 942 WP_141874722.1 ABC transporter permease -
FK543_RS13060 2472450..2473463 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FK543_RS13065 2473481..2474626 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
FK543_RS13070 2474871..2476277 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
FK543_RS13075 2476356..2476772 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FK543_RS13080 2476818..2476994 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FK543_RS13085 2477216..2477446 + 231 WP_000494244.1 YncJ family protein -
FK543_RS13090 2477538..2479499 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FK543_RS13095 2479572..2480108 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FK543_RS13100 2480161..2481375 + 1215 WP_001348055.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T127992 WP_000813794.1 NZ_CP041302:c2476994-2476818 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T127992 NZ_CP041302:c2476994-2476818 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT127992 WP_001270286.1 NZ_CP041302:c2476772-2476356 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT127992 NZ_CP041302:c2476772-2476356 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References