Detailed information of TA system TA127981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1289213..1289838
• Replicon: chromosome
• Accession: NZ_CP041302
• Organism: Escherichia coli strain MSHS 472

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FK543_RS07295
• Protein ID: WP_000911330.1
• Coordinates: 1289440..1289838 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FK543_RS07290
• Protein ID: WP_000450524.1
• Coordinates: 1289213..1289440 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK543_RS07265	1285017..1285487	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
FK543_RS07270	1285487..1286059	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FK543_RS07275	1286205..1287083	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FK543_RS07280	1287100..1288134	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
FK543_RS07285	1288347..1289060	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FK543_RS07290	1289213..1289440	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FK543_RS07295	1289440..1289838	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FK543_RS07300	1289985..1290848	+	864	WP_023277630.1	neutral zinc metallopeptidase	-
FK543_RS07305	1290863..1292878	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
FK543_RS07310	1292952..1293650	+	699	WP_141874688.1	esterase	-
FK543_RS07315	1293731..1293931	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T127981 WP_000911330.1 NZ_CP041302:1289440-1289838 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T127981 NZ_CP041302:1289440-1289838 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT127981 WP_000450524.1 NZ_CP041302:1289213-1289440 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT127981 NZ_CP041302:1289213-1289440 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33