Detailed information of TA system TA127970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4426870..4427465
• Replicon: chromosome
• Accession: NZ_CP041300
• Organism: Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: FK541_RS22345
• Protein ID: WP_063815121.1
• Coordinates: 4426870..4427220 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: FK541_RS22350
• Protein ID: WP_001223213.1
• Coordinates: 4427214..4427465 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK541_RS22325	4422324..4423346	-	1023	WP_001298067.1	ABC transporter permease	-
FK541_RS22330	4423360..4424862	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
FK541_RS22335	4424995..4425951	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
FK541_RS22340	4426261..4426791	+	531	WP_000055075.1	inorganic diphosphatase	-
FK541_RS22345	4426870..4427220	-	351	WP_063815121.1	endoribonuclease toxin ChpB	Toxin
FK541_RS22350	4427214..4427465	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
FK541_RS22355	4427677..4428018	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
FK541_RS22360	4428021..4431800	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12443.37 Da
• Isoelectric Point: 6.2206

>T127970 WP_063815121.1 NZ_CP041300:c4427220-4426870 [Escherichia coli O1:H42]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVASITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T127970 NZ_CP041300:c4427220-4426870 [Escherichia coli O1:H42]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCTCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT127970 WP_001223213.1 NZ_CP041300:c4427465-4427214 [Escherichia coli O1:H42]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT127970 NZ_CP041300:c4427465-4427214 [Escherichia coli O1:H42]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: L4JJX7