Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3762415..3763033 Replicon chromosome
Accession NZ_CP041300
Organism Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FK541_RS19345 Protein ID WP_001291435.1
Coordinates 3762815..3763033 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FK541_RS19340 Protein ID WP_000344800.1
Coordinates 3762415..3762789 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK541_RS19330 3757503..3758696 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FK541_RS19335 3758719..3761868 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FK541_RS19340 3762415..3762789 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FK541_RS19345 3762815..3763033 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FK541_RS19350 3763205..3763756 + 552 WP_000102574.1 maltose O-acetyltransferase -
FK541_RS19355 3763872..3764342 + 471 WP_022645280.1 YlaC family protein -
FK541_RS19360 3764506..3766056 + 1551 WP_022645279.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FK541_RS19365 3766098..3766451 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FK541_RS19375 3766830..3767141 + 312 WP_000409911.1 MGMT family protein -
FK541_RS19380 3767172..3767744 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T127967 WP_001291435.1 NZ_CP041300:3762815-3763033 [Escherichia coli O1:H42]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T127967 NZ_CP041300:3762815-3763033 [Escherichia coli O1:H42]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT127967 WP_000344800.1 NZ_CP041300:3762415-3762789 [Escherichia coli O1:H42]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT127967 NZ_CP041300:3762415-3762789 [Escherichia coli O1:H42]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References