Detailed information of TA system TA127965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3321661..3322366
• Replicon: chromosome
• Accession: NZ_CP041300
• Organism: Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: FK541_RS17170
• Protein ID: WP_000539521.1
• Coordinates: 3321661..3322047 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FK541_RS17175
• Protein ID: WP_001280945.1
• Coordinates: 3322037..3322366 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK541_RS17150	3317665..3318291	+	627	WP_001295292.1	glutathione S-transferase GstB	-
FK541_RS17155	3318288..3319403	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
FK541_RS17160	3319514..3319897	-	384	WP_000497137.1	biofilm formation regulator BssR	-
FK541_RS17165	3320110..3321435	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
FK541_RS17170	3321661..3322047	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FK541_RS17175	3322037..3322366	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
FK541_RS17180	3322436..3323764	-	1329	WP_022645414.1	GGDEF domain-containing protein	-
FK541_RS17185	3323772..3326119	-	2348	Protein_3113	EAL domain-containing protein	-
FK541_RS17190	3326296..3327207	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T127965 WP_000539521.1 NZ_CP041300:3321661-3322047 [Escherichia coli O1:H42]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T127965 NZ_CP041300:3321661-3322047 [Escherichia coli O1:H42]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT127965 WP_001280945.1 NZ_CP041300:3322037-3322366 [Escherichia coli O1:H42]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT127965 NZ_CP041300:3322037-3322366 [Escherichia coli O1:H42]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6